Review article: Fish sperm quality and assessment methods
DOI:
https://doi.org/10.15359/revmar10-1.5Keywords:
Sperm quality, assessment, methods, fish, reproductionAbstract
Aspects such as anthropic effects, loss of biodiversity, species displacement, introduction of exotic species, and demographic growth have caused an increase in crop yield, recovery of impacted sites, reintroduction of species and the search for alternatives to understand and evaluate reproduction of native fish species. In such context, knowing and using both female and male gametes for massive larvae production creates the need to use methodologies that would allow sorting the brood stock with genetic and physiological traits that would improve the quantity and quality of larvae to be used for massive culture, restocking, breeding production, and experiments on basic and applied sciences. This bibliographic review mentions the particularities of fish sperm cells and methodological alternatives to assess quality, by understanding activation physiology (motility percentages and times, type of movement and sperm number), including the structural analysis at extracellular and intracellular levels (spermatic morphology, damage to the membrane, comet assay, live/dead/apoptotic cells, which depend on the main objectives of reproduction or experimental programs). The development of automated cytometry and unicellular genomics equipment has allowed for a better understanding of the functioning of spermatic cells, genetic expression linked to cellular qualities and their biological implication.
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