Concentration and maximum exposure time of Lutjanus guttatus juveniles to Syzygium aromaticum eugenol
DOI:
https://doi.org/10.15359/revmar.11-1.1Keywords:
Lutjanus guttatus,, anesthetic, eugenol oil, efficient dose, time of no returnAbstract
The most efficient doses of eugenol oil from Syzygium aromaticum for surgical sedation in Lutjanus guttatus juveniles were defined, and the minimum exposure time of no return was determined as a source of information to have a safe time range when handling fish during routine work. A total of 76 juveniles produced in January 2016 with an average weight of 21.61 ± 2.0 g were selected at the Aquaculture and Marine Biotechnology Laboratory of the Marino del Pacífico Park (Gulf of Nicoya). Juveniles were exposed to 25, 100, 175, and 250 mg/L eugenol concentrations in 30 L tanks, and their induction of anesthesia and recovery were monitored. Once the most efficient dose was defined, the maximum exposure time was determined. Four times of induction were defined for this study: 4, 8, 12, and 16 min, and the recovery threshold was established at 10 min. Eugenol was determined to be an effective anesthetic for rose snapper juveniles with temperatures ranging between 24.0 and 24.8°C. The 25 mg/L concentration was sufficient for partial induction of fish and appropriate to perform less complex routine activities in the handling of snappers, while the most efficient concentration was 100 mg/L, with exposure times no greater than 8 minutes after total anesthesia.
References
Anderson, W., McKinley, R. & Colavecchia, M. (1997). The use of clove oil as an anaesthetic for rainbow trout and its effects on swimming performance. N. Am. J. Fish. Manage., 17, 301-307. https://doi.org/10.1577/1548-8675(1997)017<0301:TUOCOA>2.3.CO;2
Abdo-de la Parra, M., Rodríguez-Ibarra, L., Rodríguez-Montes de Oca, G., Velasco-Blanco, G. & Ibarra-Castro, L. (2015). Estado actual de la larvicultura del pargo flamenco (Lutjanus guttatus). Lat Am. J. Aquat. Res., 43(3), 415-423.
Barata, M., Soares, F., Aragao, C., Almeida, A., Pousão-Ferreira, P. & Ribeiro, L. (2016). Efficiency of 2-phenoxyethanol and Clove Oil for Reducing Handling Stress in Reared Meagre, Argyrosomus regius (Pisces: Sciaenidae). J. World Aquacult. Soc., 47(1), 82-92. https://doi.org/10.1111/jwas.12245
Barrantes-Elizondo, A., Álvarez-Corrales, J., Otárola-Fallas, A. & Ramírez-Mixter, O. (2016). Análisis de la situación actual del sector acuícola en Costa Rica. San José, Costa Rica: Organización de las Naciones Unidas para la Alimentación y la Agricultura.
Cárdenas, C., Toni, C., Martos‐Sitcha, J., Cárdenas, S., Heras, V., Baldisserotto, B., Heinzmann, B., Vázquez, R. & Mancera, J. M. (2016). Effects of clove oil, essential oil of Lippia alba and 2‐phe anaesthesia on juvenile meagre, Argyrosomus regius (Asso, 1801). J. Appl. Ichthyol., 32(4), 693-700. https://doi.org/10.1111/jai.13048
Chacón-Guzmán J. (2010). Efectos de la tecnología de cultivo del pargo manchado Lutjanus guttatus (Steindachner, 1869) en jaulas flotantes en el Golfo de Nicoya, Costa Rica. Tesis de maestría no publicada, Universidad Nacional, Heredia, Costa Rica.
Cho, G. K. & Heath, D. D. (2000). Comparison of tricaine methanesulphonate (MS222) and clove oil anaesthesia effects on the physiology of juvenile Chinook salmon Oncorhynchus tshawytscha (Walbaum). Aquac. Res., 31, 537,546. https://doi.org/10.1046/j.1365-2109.2000.00478.x
Cooke, S. J., Suski, C. D., Ostrand, K. G., Tufts, B. L. & Wahl, D. H. (2004). Behavioural and physiological assesment of low concentrations of clove oil anaesthetic for handling and transporting largemouth bass (Micropterus salmoides). Aquaculture, 239, 509-529. https://doi.org/10.1016/j.aquaculture.2004.06.028
Coyle, S., Durborow, R. & Tidwell, J. (2004). Anesthetics in aquaculture. Texas, EE. UU.: Southern Regional Aquaculture Center.
Cunha, F. & Rosa, I. (2006). Anaesthetic effects of clove oil on seven species of tropical reef teleosts. J. Fish. Biol., 69, 1504-1512. https://doi.org/10.1111/j.1095-8649.2006.01213.x
Cup, A. R., Hartleb, C., F., Fredricks, K. & Gaikowski, M. P. (2014). Effectiveness of eugenol sedation to reduce the metabolic rates of cool and warm water fish al high loading densities. Aquacult. Res., 47(1), 234-242. https://doi.org/10.1111/are.12485
Din, A. (2006). Molluscicidal effect of three monoterpenes oils on schistosomiasis and fascioliasis vector snails. J. Egypt. Soc. Parasitol., 36(2), 599-612.
Di Rienzo J. A., Casanoves F., Balzarini M. G., Gonzalez L., Tablada M. & Robledo C. W. (2008). InfoStat. Buenos Aires, Argentina. Grupo InfoStat, FCA.
Fischer, W., Krupp, F., Schneider, W., Sommer, C., Carpenter, K. E. & Niem, V. H. (1995). Guía FAO para la identificación de especies para los fines de la pesca. Roma, Italia: Organización de las Naciones Unidas para la Alimentación.
García-Gómez, A., De la Gándara, F. & Raja, T. (2002). Utilización del aceite de clavo, Syzygium aromaticum L. (Merr. & Perry), como anestésico eficaz y económico para labores rutinarias de manipulación de peces marinos cultivados. Bol. Inst. Español Oceanogr., 18(1-4), 21-23.
González-Escobar, R. (2002). Eugenol: propiedades farmacológicas y toxicológicas. Ventajas y desventajas de su uso. Rev. Cubana. Estomatol., 39(2), 139-156.
Grush, J., Noakes, D. & Morcia, R. (2004). The efficacy of clove oil as an anesthetic for the zebrafish, Danio rerio (Hamilton). Zebrafish, 1(1), 46-53. https://doi.org/10.1089/154585404774101671
Guénette, S., Uhland, F., Hélie, P., Beaudry, F. & Vachon, P. (2007). Pharmacokinetics of eugenol in rainbow trout (Onchorhynchus mykiss). Aquaculture, 266, 262-265. https://doi.org/10.1016/j.aquaculture.2007.02.046
Gullian, M. & Villanueva, J. (2009). Efficacy of tricaine methanesulphonate and clove oil as anaesthetics for juvenile cobia Rachycentron canadum. Aquac. Res., 40(7), 852-860. https://doi.org/10.1111/j.1365-2109.2009.02180.x
Herrera-Ulloa, A., Chacón-Guzmán, J., Zúñiga-Calero, G., Fajardo-Espinoza, O. & Jiménez-Montealegre, R. (2009). Acuicultura de pargo la mancha Lutjanus guttatus (Steindachner, 1869) en Costa Rica dentro de un enfoque sistémico. Rev. Cien. Mar. Cost., 1, 197-213.
Hoskonen, P. & Pirhonen, J. (2004). Temperature effects on anaesthesia with clove oil in sex temperate-zone fishes. J. Fish Biol., 64, 1136-1142. https://doi.org/10.1111/j.1095-8649.2004.00359.x
Hussein, M., Wada, S., Hatai, K. & Yamamoto, A. (2000) Antimycotic activity of eugenol against selected water molds. J. Aquat. Anim. Health, 12, 224-229. https://doi.org/10.1577/1548-8667(2000)012<0224:AAOEAS>2.0.CO;2
Iversen, M., Finstand, B., Mackinley, R. & Eliassen, R. (2003). The efficacy of metomidate, clove oil, Aqui-S and Benzoak® as anaesthetics in Atlantic salmon (Salmo salar L.) smolts, and their potential stress-reducing capacity. Aquaculture, 221, 549-566. https://doi.org/10.1016/S0044-8486(03)00111-X
Javahery, S., Nekoubin, H. & Moradlu, A. (2012). Effect of anasthesia with clove oil in fish (review). Fish Physiol. Biochem., 38(6), 1545-1552. https://doi.org/10.1007/s10695-012-96825
Marín-Méndez, G., Torres-Cortés, A., Naranjo-Suarez, L., Chacón-Novoa, R. & Rondón-Barragan, L. (2012). Concentración letal 50 a 96 horas de eugenol en cachama blanca (Piaractus brachyopomus). Orinoquia, 16(2), 62-66.
Millán-Ocampo, L., Torres-Cortés, A., Marín-Méndez, G. A., Ramírez-Duart, W., Vásquez-Piñeros, M. A. & Rondón-Barragán, I. (2012). Concentración anestésica del eugenol en peces escalares (Pterophyllum scalare). Rev. Investig. Vet. Peru, 23(2), 171-181.
Mylonas, C. C., Cardinaletti, G., Sigelaki, I. & Polzonetti-Magni, A. (2005). Comparative efficacy of clove oil and 2-phenoxyethanol as anesthetics in the aquaculture of European sea bass (Dicentrarchus labrax) and gilthead sea bream (Sparus aurata) at different temperatures. Aquaculture, 246(1-4), 467-481. https://doi.org/10.1016/j.aquaculture.2005.02.046
Oliveira, L., Branco, R., Luscher, A., Lira, A., Rocha, T. & Santos, G. (2008). Eugenol como anestésico para a tilapia-do-nilo. Pesq. Agropec. Bras., 43, 1069-1074. http://dx.doi.org/10.1590/S0100-204X2008000800017
Park, I., Lee, S., Kim, Y., Park, K., Hur, J., Yoo, J. & Song, Y. (2003). Anesthetic effect of lidocaine hydrochloridesodium bicarbonate and MS-222 on the greenling (Hexagrammos otakii). J. Korean Fish. Soc., 39(8), 449-453. http://dx.doi.org/10.5657/kfas.2003.36.5.449.
Park, M., Hur, W., Im, S., Seol, D., Lee, J. & Park, I. (2008). Anaesthetic efficacy and physiological responses to clove oil anaesthetized kelp grouper Epinephelus bruneus. Aquat. Res., 39, 877-884. https://doi.org/10.1111/j.1365-2109.2008.01941.x
Perry, S. & Gilmour, K. (2006) Acid-base balance and CO2 excretion in fish: unanswered questions and emerging models. Resp. Physiol. Neuro., 154, 199-215. https://doi.org/10.1016/j.resp.2006.04.010
Portz, D., Woodley, C. & Cech, C. (2006) Stress-associated impacts of short-term holding on fishes. Rev. Fish. Biol. Fish , 16, 125-170. https://doi.org/10.1007/s11160-006-9012-z
Prashar, A., Locke, I. C. & Evans, C. S. (2006). Cytotoxicity of clove (Syzygium aromaticum) oil and its major components to human skin cells. Cell Proliferation, 39(4), 241-248. https://doi:10.1111/j.1365-2184.2006.00384.x
Prince, A. & Powell, C. (2000). Clove oil as an anesthetic for invasive field procedures on adult rainbow trout. North Am. J. Fish. Manage., 20, 1029-1032. https://doi.org/10.1577/1548-8675(2000)020<1029:COAAAF>2.0.CO;2
Roo, J., Fernández‐Palacios, H., Hernández‐Cruz, C. M., Mesa‐Rodriguez, A., Schuchardt, D. & Izquierdo, M. (2014). First results of spawning and larval rearing of longfin yellowtail Seriola rivoliana as a fast‐growing candidate for European marine finfish aquaculture diversification. Aquat. Res., 45(4), 689-700. https://doi.org/10.1111/are.12007
Roubach, R., Gomes, L.C. & Val, A.L. (2001). Safest level of tricaine mehanesulfonate (MS-222) to induce anesthesia in juveniles matrinx, Brycon cephalus. Acta Amazon, 31, 159-163. http://dx.doi.org/10.1590/1809-43922001311163
Roubach, R., Gómez, L., Fonseca, F. & Val, A. (2005). Eugenol as na efficacious anaesthetic for tambaqui, Colossoma macropomum (Cuvier). Aquat. Res., 36(11), 1056-1061. https://doi.org/10.1111/j.1365-2109.2005.01319.x
Sladky, K. K., Swanson, C. R., Stoskopf, M. K., Loomis, M. R. & Lewbart, G. A. (2001). Comparative efficacy of tricaine methanesulfonate and clove oil for use as anesthetic in red pacu (Piaractus brachypomus). Am. J. Vet. Res., 62, 337-342. https://doi.org/10.2460/ajvr.2001.62.337
Small, B. (2003). Anesthetic efficacy of metomidate and comparison of plasma cortisol responses to tricaine methanesulfonate, quinaldine and clove oil anesthetized channel catfish Ictalurus punctatus. Aquaculture, 218, 177-185. https://doi.org/10.1016/S0044-8486(02)00302-2
Soto, C. & Burhanuddin, C. (1995). Clove oil as a fish anaesthetic for measuring length and weight of rabbitfish (Siganus lineatus). Aquaculture, 136, 149-152. https://doi.org/10.1016/0044-8486(95)01051-3
Souza, M., Vettorazzi, M., Kobayashi, R., Neto, F. & de Andrade, M. (2015). Eugenol as an anaesthetic in the management of farmed lane snapper, Lutjanus synagris (Linnaeus, 1758). Rev. Cien. Agron., 46(3), 532-538. http://dx.doi.org/10.5935/1806-6690.20150035
Steindachner, F. (1869). Ichthyologische Notizen. Sitzber. Akad., 60, 290-318.
Summerfelt, R. C. & Smith, L. S. (1990). Anaesthesia, surgery and related techniques. In C. B. Schreck & P. B. Moyle (Eds.), Methods in Fish Biology (pp. 213-272). Maryland, EE. UU.: American Fisheries Society.
Taylor, P. W. & Roberts, S. D. (1999). Clove oil: an alternative anaesthetic for aquaculture. N Am. J. Aquact., 61, 150-155.
UFR (Use of Fishes in Research) Committee. (2013). Guidelines for the use of fishes in research. Bethesda, Maryland: American Fisheries Society.
Velísek, J., Wlasow, T., Gomulka, P., Svobodová, Z., Novotn, L. & Ziomek, E. (2006). Effects of clove oil anaesthesia on European Catfish (Silurus glanis L.). Acta Veterinaria Brno., 75, 99-106. https://doi.org/10.2754/avb200675010099
Walsh, C. T. & Pease, B. C. (2002). The use of clove oil as an anaesthetic for the longfinned eel, Anguilla reinhardtii (Steindachner). Aquat. Res., 33, 627-635. https://doi.org/10.1046/j.1365-2109.2002.00701.x
Webber, R. A., Peleteiro, J., García-Martín, L. & Aldegunde, M. (2009). The efficacy of 2-phenoxyethanol, metomidate, clove oil and MS-222 as anaesthetic agents in the Senegalese sole (Solea senegalensis Kaup 1858). Aquaculture, 288, 147-150. https://doi.org/10.1016/j.aquaculture.2008.11.024
Woody, C. A., Nelson, J. & Ramstad, K. (2002). Clove oil as an anaesthetic for adult sockeye salmon: field trials. J. Fish. Biol., 60, 340-347. https://doi.org/10.1111/j.1095-8649.2002.tb00284.x
Zahl, I. H., Samuelsen, O. & Kiessling, A. (2012). Anaesthesia of farmed fish: implications for welfare. Fish. Physiol. Biochem., 38, 201-218. https://doi.org/10.1007/s10695-011-9565-1
Downloads
Published
How to Cite
Issue
Section
License
General terms and conditions
Revista Ciencias Marinas y Costeras by Universidad Nacional is located under a Licencia Creative Commons Atribución-NoComercial-SinDerivadas 3.0 Costa Rica.
The journal is hosted in open-access repositories such as the Repositorio Institucional de la Universidad Nacional, the Repositorio Kimuk de Costa Rica and la Referencia.
The editorial source of the journal must be acknowledged. For this purpose, use the doi identifier of the publication.
Self-archiving policy: The journal allows the self-archiving of articles in their refereed version, edited and approved by the Editorial Board of the Journal so that they are available in Open Access through the Internet. More information at the following link: https://v2.sherpa.ac.uk/id/publication/28915
