BACILLUS SPECIES IN THE OCEANIC WATERS ADJACENT TO CUBA: ASSOCIATION BETWEEN THEIR DISTRIBUTION AND METABOLIC ACTIVITY
DOI:
https://doi.org/10.15359/revmar.2.5Keywords:
Bacillus, distribution, metabolic activity, oceanic waters, CubaAbstract
The aim of the present work was to identify spore-forming Gram-positive Bacillus strains isolated from oceanic waters adjacent to Cuba and to establish a possible relationship between their distribution and the metabolic activity of the isolates. Total protein patterns, derived from SDS-PAGE, were used to build a non-rooted dendrogram where the strains appeared clustered in three nodes. No direct relationship was observed between a node and particular species. In contrast, according to the physical and chemical characteristics of the zones, node I was different from node II and III, since it comprises strains from the farthest zones of the coast, poorer in nutrients. On the other hand, nodes II and III mainly collect strains isolated from nutrient and organic matter-enriched zones. Associating the node clustering with metabolic activities, it was found that in node I the ratio: number of positive activities/strain was 2.3, followed by node II with a ratio of 3.3, and finally node III exhibiting a ratio equal to 3.7. This could suggest that different total protein patterns in bacteria belonging to the same specie, but coming from environments with different degree of nutrient richness, could be an indicator of the capacities of these microorganisms to adapt and live in different environments.
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