CHEMICAL AND FUNCTIONAL CHARACTERIZATION OF ANTIMICROBIAL METABOLITES ISOLATED FROM THE ASCIDIAN RHOPALAEA BIRKELANDI

Authors

  • Maribel Cordero Universidad Nacional, Costa Rica
  • Henry Borbón Universidad Nacional, Costa Rica
  • Félix R. Román Universidad de Puerto Rico - Recinto de Río Piedras, Puerto Rico
  • Luis Morell Universidad de Puerto Rico - Recinto de Río Piedras, Puerto Rico
  • Rigoberto Víquez Universidad Nacional, Costa Rica
  • Luis R. Villegas Universidad Nacional, Costa Rica
  • Roy Soto Universidad Nacional, Costa Rica
  • Ilena Vega Universidad Nacional, Costa Rica

DOI:

https://doi.org/10.15359/revmar.3.9

Keywords:

Antibiotic activity, ascidians, biological activity, bioactive extracts

Abstract

Marine ecosystems have a very large diversity of resources, most of them still partially unknown, and a few others exploited for development of new industrial and toxicological products. The objective of this investigation was to determine whether the acetone extract of the ascidia R. birkelandi from the Pacific coast of Costa Rica showed qualitative antimicrobial activity against the S. aureus bacteria and the G. candidum fungus, and to verify their main secondary metabolites in the active extract using chromatographic and spectroscopic techniques. Ascidians were collected at Tambor, Guanacaste, Costa Rica, between December 2007 and March 2008. Activity against the Gram positive bacteria and fungi was evaluated using ethanolic (95%) and acetonic extracts. Both extracts showed activity against G. candidum; however, only the acetonic extract showed activity against S. aureus. A coumarin and a hydroxyanthraquinone were isolated from a crude extract of R. birkelandi as metabolites present in the active fraction. Purification and isolation were performed by chromatographic techniques and solid phase extraction. Structural information was obtained by spectroscopic analyses: Ultraviolet (UV-Visible), Fourier Transform Infrared (FT-IR), Liquid Chromatography Tandem Mass Spectrometry (LC/MS/MS), Gas Chromatography Mass Spectrometry (GC/MS), Proton Nuclear Magnetic Resonance (1HNMR), and Carbon-13 Magnetic Resonance (13C-NMR). Further studies are recommended for characterization and quantification of the active components of this extract and the possible elucidation of the mechanisms of action.

Author Biographies

Maribel Cordero, Universidad Nacional

Laboratorio de Investigación y Desarrollo en Tecnología Química, Cátedra de Química Orgánica

Henry Borbón, Universidad Nacional

Laboratorio de Investigación y Desarrollo en Tecnología Química, Cátedra de Química Orgánica

Félix R. Román, Universidad de Puerto Rico - Recinto de Río Piedras

Departamento de Química

Luis Morell, Universidad de Puerto Rico - Recinto de Río Piedras

Departamento de Química

Rigoberto Víquez, Universidad Nacional

Programa de Maestría en Ciencias Marinas y Costeras

Luis R. Villegas, Universidad Nacional

Laboratorio de Investigación y Desarrollo en Tecnología Química, Cátedra de Química Orgánica

Roy Soto, Universidad Nacional

Laboratorio de Investigación y Desarrollo en Tecnología Química, Cátedra de Química Orgánica

Ilena Vega, Universidad Nacional

Laboratorio de Investigación y Desarrollo en Tecnología Química, Cátedra de Química Orgánica

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Published

2011-12-31

How to Cite

Cordero, M., Borbón, H., Román, F. R., Morell, L., Víquez, R., Villegas, L. R., Soto, R., & Vega, I. (2011). CHEMICAL AND FUNCTIONAL CHARACTERIZATION OF ANTIMICROBIAL METABOLITES ISOLATED FROM THE ASCIDIAN RHOPALAEA BIRKELANDI. Revista Ciencias Marinas Y Costeras, 3, 111-125. https://doi.org/10.15359/revmar.3.9

Issue

Section

Scientific articles

How to Cite

Cordero, M., Borbón, H., Román, F. R., Morell, L., Víquez, R., Villegas, L. R., Soto, R., & Vega, I. (2011). CHEMICAL AND FUNCTIONAL CHARACTERIZATION OF ANTIMICROBIAL METABOLITES ISOLATED FROM THE ASCIDIAN RHOPALAEA BIRKELANDI. Revista Ciencias Marinas Y Costeras, 3, 111-125. https://doi.org/10.15359/revmar.3.9

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