Evaluation of Escherichia coli concentrations in Crassostrea gigas and seawater in two oyster growing areas in the Gulf of Nicoya, Costa Rica
DOI:
https://doi.org/10.15359/revmar.6.11Keywords:
Marine pollution, mariculture, Escherichia coli, Crassostrea gigas, Costa RicaAbstract
Costa Rica does not have references on the health quality of Crassostrea gigas growing areas, which can pose a potential public health risk. This study evaluated the temporal trends of Escherichia coli concentrations in the C. gigas oyster and in the seawater of Punta Cuchillo and Punta Morales growing areas as a basis for the classification and monitoring of bivalve mollusc production sites in the Gulf of Nicoya, Costa Rica. Monthly samples of seawater and C. gigas were collected from each site from July 2011 to June 2012, and their levels of E. coli were determined using the most probable number method. Temperature and salinity were recorded at a one meter depth. Precipitation data was provided by Instituto Meteorológico Nacional (the National Meteorological Institute). Results indicated that the evaluated areas are probably being affected by wastewater discharge. Punta Cuchillo and Punta Morales could be classified as class A or authorized areas for the production of bivalve molluscs. A significant seasonal variability of E. coli concentrations was determined in seawater and oysters from the Gulf of Nicoya, defined primarily by salinity. National legislation should be created to establish controls and implement a monitoring system ensuring the bacteriological quality of the areas used for the cultivation of bivalve molluscs in the country.References
Acuña, J. A., García, V. & Mondragón, J. (1998). Comparación de algunos aspectos fisicoquímicos y calidad sanitaria del Estero de Puntarenas, Costa Rica. Rev. Biol. Trop., 46(Suppl. 6), 1-10.
Almeida, C. & Soares, F. (2012). Microbiological monitoring of bivalves from the Ria Formosa Lagoon (south coast of Portugal): a 20 years of sanitary survey. Mar. Pollut. Bull., 64(2), 252-262. doi: http://dx.doi.org/10.1016/j.marpolbul.2011.11.025
Anacleto, P., Pedro, S., Nunes, M. L., Rosa, R. & Marques, A. (2013). Microbiological composition of native and exotic clams from Tagus estuary: effect of season and environmental parameters. Mar. Pollut. Bull., 74(1), 116-124. doi: http://dx.doi.org/10.1016/j.marpolbul.2013.07.019
Barrera-Escorcia, G. & Namihira-Santillán, P. E. (2004). Contaminación microbiológica en la zona costera de Akumal, Quintana Roo, Mexico. Hidrobiológica, 14(1), 27-35.
Barrera-Escorcia, G., Wong-Chang, I., Sobrino-Figueroa, A. S., Guzmán-García, X. & Saavedra-Villeda, F. (1999). Evaluación microbiológica de la laguna de Tamiahua, Veracruz, en el ciclo 1994-1995. Hidrobiológica, 9(2), 125-134.
Bettencourt, F., Almeida, C., Santos, M. I., Pedroso, L. & Soares, F. (2013). Microbiological monitoring of Ruditapes decussatus from Ria Formosa Lagoon (South of Portugal). J. Coast. Conserv., 17(3), 653-661.doi: http://dx.doi.org/10.1007/s11852-013-0264-1
Campos, C. J. A., Acornley, R., Morgan, O. C. & Kershaw, S. (2013a). Trends in the levels of Escherichia coli in commercially harvested bivalve shellfish from England and Wales, 1999-2008. Mar. Pollut. Bull., 67(1-2), 223-227. doi: http://dx.doi.org/10.1016/j.marpolbul.2012.11.030
Campos, C. J. A., Kershaw, S. R. & Lee, R. J. (2013b). Environmental influences on faecal indicator organisms in coastal waters and their accumulation in bivalve shellfish. Estuaries Coasts, 36(4), 834-853. doi: http://dx.doi.org/10.1007/s12237-013-9599-y
Clesceri, L. S., Greenberg, A. E. & Trussell, R. R. (1989). Standard methods for the examination of water and wastewater (17th ed.). Washington, USA.: American Public Health Association, American Water Works Association, Water Pollution Control Federation.
Derolez, V., Soudant, D., Fiandrino, A., Cesmat, L. & Serais, O. (2013). Impact of weather conditions on Escherichia coli accumulation in oysters of the Thau lagoon (the Mediterranean, France). J. Appl. Microbiol., 114(2), 516-525. doi: http://dx.doi.org/10.1111/jam.12040
Downes, F. P. & Ito, K. (2001). Compendium of methods for the microbiological examination of foods (4th ed.). Washington, USA.: American Public Health Association. doi: http://dx.doi.org/10.2105/9780875531755
García, V., Acuña-González, J., Vargas-Zamora, J. A. & García-Céspedes, J. (2006). Calidad bacteriológica y desechos sólidos en cinco ambientes costeros de Costa Rica. Rev. Biol. Trop., 54(Supl. 1), 35-48.
Halpern, B. S., Walbridge, S., Selkoe, K. A., Kappel, C. V., Micheli, F., D’Agrosa, C. ... Watson, R. (2008). A global map of human impact on marine ecosystems. Science, 319(5865), 948-952. doi: http://dx.doi.org/10.1126/science.1149345
IMN. Instituto Meteorológico Nacional. (2014). Clima en Costa Rica. Instituto Meteorológico Nacional de Costa Rica. Recovered January 22, 2014, retrieved from http://www.imn.ac.cr/educacion/CLIMADECOSTARICA.html
Lee, R. J. & Silk, R. (2013). Sources of variation of Escherichia coli concentrations in bivalve molluscs. J. Water Health, 11(1), 78-83. doi: http://dx.doi.org/10.2166/wh.2012.114
Lee, R., Lovatelli, A. & Ababouch, L. (2008). Bivalve depuration: fundamental and practical aspects. Rome, Italy: FAO Fisheries Technical Paper.
Martínez, D. I. & Oliviera, A. J. F. C. (2010). Faecal bacteria in Perna perna (Linnaeus, 1758) (Mollusca: Bivalvia) for biomonitoring coastal waters and seafood quality. Braz. J. Oceanogr., 58, 29-35. doi: http://dx.doi.org/10.1590/S1679-87592010000700005
Mignani, L., Barbieri, E., Marques, H. L. A. & Oliviera, A. J. F. C. (2013). Coliform density in oyster culture waters and its relationship with environmental factors. Pesq. Agropec. Bras., 48(8), 833-840. doi: http://dx.doi.org/10.1590/S0100-204X2013000800004
Oliveira, J., Cunha, A., Castilho, F., Romalde, J. L. & Pereira, M. J. (2011). Microbial contamination and purification of bivalve shellfish: crucial aspects in monitoring and future perspectives - a mini-review. Food Control, 22(6), 805-816. doi: http://dx.doi.org/10.1016/j.foodcont.2010.11.032
Peterson, C. L. (1960). The physical oceanography of the Gulf of Nicoya, Costa Rica, a tropical estuary. Inter-Amer. Trop. Tuna Comm. Bull., 4(4), 137-216.
R Core Team. (2013). R: A language and environment for statistical computing. Viena, Austria: R Foundation for Statistical Computing.
Soegianto, A. & Supriyanto, A. (2008). Concentration of pathogenic bacteria and trace metals in bivalve mollusk Anadara granosa (Bivalvia: Arcidae) harvested from East Java Coast, Indonesia. Cah. Biol. Mar., 49, 201-207.
Šolić, M. & Krstulović, N. (1992). Separate and combined effects of solar radiation, temperature, salinity, and pH on the survival of fecal coliforms in seawater. Mar. Pollut. Bull., 24(8), 411-416. doi: http://dx.doi.org/10.1016/0025-326X(92)90503-X
Vega, C. L. A., Marín, V. C., Pacheco, P. O. & Zúñiga, C. G. (2013). Preliminary assessment of small scale bacterial depuration of Crassostrea gigas and Anadara spp., Gulf of Nicoya, Costa Rica. Rev. Mar. Cost., 5, 107-117.
Voorhis, A. D., Epifanio, C. E., Maurer, D., Dittel, A. I. & Vargas, J. A. (1983). The estuarine character of the Gulf of Nicoya, an embayment on the Pacific coast of Central America. Hydrobiologia, 99, 225-237. doi: http://dx.doi.org/10.1007/BF00008774
Younger, A. D. & Reese, R. A. (2013). Comparison of Escherichia coli levels between bivalve mollusc species across growing sites in England and Wales. J. Shellfish Res., 32(2), 527-532. doi: http://dx.doi.org/10.2983/035.032.0232
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