Influência de xenobióticos na luminescência de cepas de Vibrio harveyi isoladas das águas marinhas cubanas
DOI:
https://doi.org/10.15359/revmar.16-2.2Palabras clave:
bactérias, bioensaio, contaminação, luminescência, xenobióticosResumen
Os testes de toxicidade usando bactérias luminescentes são ferramentas promissoras para avaliar a qualidade dos ambientes aquáticos, devido à sua alta sensibilidade aos poluentes. Nesta pesquisa, o efeito de xenobióticos de diferentes naturezas: sais de mercúrio, cobre, cromo, prata, ferro e quatro pesticidas, sobre a luminescência de cepas de Vibrio harveyi CBM-784, CBM-976 e CBM-992, isoladas das águas da plataforma cubana. As cepas selecionadas mostraram uma redução da emissão de luminescência após 15 minutos de exposição aos compostos testados com uma sequência decrescente de toxicidade, consistente para as três cepas da seguinte forma: HgCl2 > CuSO4 ≈ Cuproflow > K2Cr2O7 > Sphere Max ≈ Kospi-sc 130 > AgNO3 > Fe2(SO4)3 > Envidor. Os resultados sugerem que essas culturas poderiam ser usadas para o projeto de um biossensor de contaminação, já que a luminescência responde a concentrações nanomolares dos tóxicos testados.
Referencias
Axelrod, T., Eltzov, E. & Marks, R. S. (2016). Bioluminescent bioreporter pad biosensor for monitoring water toxicity. Talanta 149, 290-297. https://doi.org/10.1016/j.talanta.2015.11.067
Ayangbenro, A. S. & Babalola, O. O. (2017). A new strategy for heavy metal polluted environments: A review of microbial biosorbents. Int. J. Environ. Res. Public Health, 14(94), 1-16. https://doi.org/10.3390/ijerph14010094
Balali-Mood, M., Naseri, K., Tahergorabi, Z., Khazdair, M. R. & Sadeghi, M. (2021). Toxic Mechanisms of Five Heavy Metals: Mercury, Lead, Chromium, Cadmium, and Arsenic. Front. Pharmacol., 13(13), 643972. https://doi.org/10.3389/fphar.2021.643972
Baumann, P. & Baumann, L. (1981). The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, & Alcaligenes. The Prokaryotes. A handbook on habitats, isolation, & identification of bacteria. Berlin. Springer-Verlag.
Braun, V. & Hantke, K. (2007). Acquisition of Iron by Bacteria. En D. H. Nies, S. Silver (Eds.), Molecular Microbiology of Heavy Metals (pp. 189-219). Berlin: Springer.
Beh, W. C., Lim, Y. K., Asmat, A., Lee, Y. H. & Salmijah, S. (2010). The potential of luminescent Bacteria “Photobacterium leiognathi” as biosensor for the detection of aquatic toxicity. Environ. Nat. Res. J., 8(3), 1-9.
Bolelli, L., Ferri, E. N. & Girotti, S. (2016). The management and exploitation of naturally light-emitting bacteria as a flexible analytical tool: A tutorial. Anal. Chim. Acta., 934, 22-35. https://doi.org/10.1016/j.aca.2016.05.038
Boynton, L. (2009). Using bioluminescent bacteria to detect water contaminants. J. U.S. SJWP., 4, 29-41.
Camanzi, L., Bolelli, M., Maiolini, E., Girotti, S. & Matteuzzi, D. (2011). Optimal conditions for stability of photoemission and freeze drying of two luminescent bacteria use in a biosensor. Environ. Toxicol. Chem., 30(4), 801-805. https://doi.org/10.1002/etc.452
Chen, B. & Dong, S. (2022). Mercury Contamination in Fish and Its Effects on the Health of Pregnant Women and Their Fetuses, and Guidance for Fish Consumption-A Narrative Review. Int. J. Environ. Res. Public Health, 19(23), 15929. https://doi.org/10.3390/ijerph192315929
Cho, J.-C., Park, K.-J., Ihmb, H.-S., Park, J.-E., Kim, S.-Y., Kang, I., … & Kim, S.-J. (2004). A novel continuous toxicity test system using a luminously modified freshwater bacterium. Biosens Bioelectron., 20(2), 338-44. https://doi.org/10.1016/j.bios.2004.02.001
Coutiño-Rodríguez, E. Md. R. & Pérez-Gutiérrez, R. A. (2007). Los compuestos de plata y la salud. Salud Comunidad, 3(5), 29-38.
de Carvalho, C. C. C. R. & Fernandes, P. (2010). Production of metabolites as bacterial responses to the marine environment. Mar. Drugs., 8(3), 705-727. https://doi.org/10.3390/md8030705
de la Gala Morales, M. (2014). Desarrollo y aplicación de nuevas tecnologías analíticas para la determinación de contaminantes ambientales (metales pesados y benzo(a)pireno). (Tesis de doctorado no publicada), Universidad de Extremadura, España.
Dupont, C. L., Grass, G. & Rensing, C. (2011). Copper toxicity and the origin of bacterial resistance-new insights and applications. Metallomics, 3(11), 1109-1118. https://doi.org/10.1039/c1mt00107h
Excel. (2018). Microsoft Corporation. Microsoft Excel. https://office.microsoft.com/excel
Fang, Z., Zhao, M., Zhen, H., Chen, L., Shi, P. & Huang, Z. (2014). Genotoxicity of Tri- and hexavalent Chromium compounds In Vivo and their modes of action on DNA damage In Vitro. PLoS One., 9(8), e103194. https://doi.org/10.1371/journal.pone.0103194
Fernández-Piñas, F., Rodea-Palomares, I., Leganés, F., González-Pleiter, M. & Muñoz-Martín, M. A. (2014). Evaluation of the Ecotoxicity of Pollutants with Bioluminescent Microorganisms. Adv. Biochem. Eng. Biotechnol., (2)145, 65-135. https://doi.org/10.1007/978-3-662-43619-6_3
Futra, D., Heng, L. Y., Surif, S., Ahmad, A. & Ling, T. L. (2014). Microencapsulated Aliivibrio fischeri in alginate microspheres for monitoring heavy metal toxicity in environmental waters. Sensors, 14, 23248-23268. https://doi.org/10.3390/s141223248
Gaetke, L. M. & Chow, C. K. (2003). Copper toxicity, oxidative stress, and antioxidant nutrients. Toxicol., 189(1-2), 147-163. https://doi.org/10.1016/S0300-483X(03)00159-8
Girotti, S., Bolelli, L., Ferri, E., Carpené, E. & Isani, G. (2015). Bioindicators in enviromental monitoring: bioluminiscen bacteria, algae and honeybees. Paper presented at the International Conference on Environmental Science and Technology, Greece.
González-Marín, B., Calderón-Segura, M. E. & Sekelsky, J. (2023). Atm/Chk2 and atr/Chk1 pathways respond to dna damage induced by Movento® 240SC and Envidor® 240SC Keto-Enol Insecticides in the Germarium of Drosophila melanogaster. Toxics, 11(9), 754. https://doi.org/10.3390/toxics11090754
Grass, G. (2007). New transport deals for old Iron. In D. H. Nies & S. Silver. (Eds.), Molecular Microbiology of Heavy Metals. Microbiology Monographs (pp. 221-233). Berlin: Springer. https://doi.org/10.1007/7171_2006_079
Gutiérrez-Corona, J. F., Espino-Saldaña, A. E., Coreño-Alonso, A., Reyna-López, G. E., Acevedo-Aguilar, F. J., Fernández, F. J., ... & Wrobel, K. (2010). Interacciones microbianas con el cromo: mecanismos y potencial biotecnológico. Rev. Latinoamb. Biotecnol Algal., 1(1), 47-63.
Halmi, M. I. E., Kassim, A. & Shukor, M. Y. (2019). Assessment of heavy metal toxicity using a luminescent bacterial test based on Photobacterium sp. strain MIE. Rend. Fis. Acc. Lincei, 30(3), 589-601. https://doi.org/10.1007/s12210-019-00809-5
Hong, G., Antaris, A. L. & Dai, H. (2017). Near-infrared fluorophores for biomedical imaging. Nat. Biomed. Eng., 1, 0010. https://doi.org/10.1038/s41551-016-0010
Jan, A. T., Azam, M., Choi, I., Ali, A. & Haq, Q. M. R. (2016). Analysis for the presence of determinants involved in the transport of mercury across bacterial membrane from polluted water bodies of India. Braz. J. Microbiol., 47(1), 55-62. https://doi.org/10.1016/j.bjm.2015.11.023
Kirsop, B. E. & Doyle, A. (Eds.). (1991). Maintenance of Microorganisms and Cultured Cells: a Manual of Laboratory Methods. 2nd Edition. EE. UU.: Academic Press.
Kurbatska, O. & Orobchenko, O. (2022). Toxicological evaluation of feeds with different levels of heavy metals using luminescent microorganisms Photobacterium рhosphoreum. Scien. Mess. LNU Vet. Med. Biotech. Ser.: Vet. Sci., 24(106), 158-167. https://doi.org/10.32718/nvlvet10624
Lerch, G. (1977). La experimentación en las Ciencias Biológicas y Agrícolas. Cuba.: Editorial Científico Técnica.
Liu, Y.-R., Lu, X., Zhao, L., Jing, A., He, J.-Z., Pierce, E. M., … & Gu, B. (2016). Effects of cellular sorption on mercury bioavailability and methylmercury production by desulfovibrio desulfuricans ND132. Environ. Sci. Technol., 50(24), 13335-13341. https://doi.org/10.1021/acs.est.6b04041
López-Roldan, R., Kazlauskaite, L., Ribo, J., Riva, M. C., González, S. & Cortina, J. L. (2012). Evaluation of an automated luminescent bacteria assay for in situ aquatic toxicity determination. Sci. Total. Environ., 440, 307-313. https://doi.org/10.1016/j.scitotenv.2012.05.043
Mackenzie, E. L., Iwasaki, K. & Tsuji, Y. (2008). Intracellular iron transport and storage: From molecular mechanisms to health implications. Antioxid. Redox. Signal., 10(6), 997-1030. https://doi.org/10.1089/ars.2007.1893
Macomber, L. & Imlay, J. A. (2009). The iron-sulfur clusters of dehydratases are Primary intracelular targets of copper toxicity. Proc. Natl. Acad. Sci. U.S.A., 106, 8344-8349. https://doi:10.1073/pnas.0812808106
Mahendran, G., Savitha, T., Khalifa, A. Y., Sharmae, A. & Sankaranarayanane, A. (2022). Evaluation of environment by microbial sensors. In P. Verma & M. P. Shah (Eds.), Bioprospecting of Microbial Diversity (pp. 407-424). EE. UU.: Elsevier. https://doi.org/10.1016/B978-0-323-90958-7.00010-8
Marrero, K. & Fando, R. (2009). Sistemas de homeostasis del cobre en las bacterias Gram negativas Escherichia coli y Vibrio cholerae. Rev. CENIC Cienc. Biol., 40(3), 186-198.
Martín, A., Serrano, S., Santos, A., Marquina, D. & Vázquez, C. (2010). Bioluminiscencia bacteriana. Reduca (Biología). Ser. Microbiol., 3(5), 75-86.
Muneeswaran, T., Kalyanaraman, N., Vennila, T., Rajesh Kannan, M. & Ramakritinan, C. M. (2021). Rapid assessment of heavy metal toxicity using bioluminescent bacteria Photobacterium leiognathi strain GoMGm1. Environ. Monit. Assess., 193(3), 109. https://doi.org/10.1007/s10661-021-08860-2
OriginPro. (2024). OriginLab Corporation. EE. UU. https://www.originlab.com
Park, J., Shin, K., Lee, H., Choi, S., Kim, G., Depuydt, S., ... & Han, T. (2023). Evaluating ecotoxicological assays for comprehensive risk assessment of toxic metals present in industrial wastewaters in the Republic of Korea. Sci. Total Environ., 1(867), 161536. https://doi.org/10.1016/j.scitotenv.2023.161536
Parvez, S., Venkataraman, C. & Mukherji, S. (2006). A review on advantages of implementing luminescence inhibition test (Vibrio fischeri) for acute toxicity prediction of chemicals. Environ. Int., 32(2), 265-268. https://doi.org/10.1016/j.envint.2005.08.022
Perego, P., Fanara, L., Zilli, M. & Borghi, M. D. (2002). Applications of luminous bacteria on environmental monitoring. Chem. Biochem. Eng. Q., 16(2), 87-92.
Ramteke, P. W., Sagar, A. & Singh, M. P. (2019). Assessment of Wastewater Toxicity by Vibrio fischeri Bioassay. Int. J. Ecol. Env. Sci., 45(1), 15-17.
Sáenz, C. I. & Nevárez, G. V. (2010). La bioluminiscencia de microorganismos marinos y su potencial biotecnológico. Acta Quim. Mex., 2(3), 1-7.
Schaefer, J. K., Szczuka, A. & Morel, F. M. M. (2014). Effect of divalent metals on Hg(II) uptake and methylation by bacteria. Environ. Sci. Technol., 48(5), 3007-30013. https://doi.org/10.1021/es405215v
Tanet, L., Tamburini, C., Baumas, C., Garel, M., Simon, G. & Casalot, L. (2019). Bacterial Bioluminescence: Light Emission in Photobacterium phosphoreum Is Not Under Quorum-Sensing Control. Front. Microbiol., 10(365), 1-9. https://doi.org/10.3389/fmicb.2019.00365
Tchounwou, P. B., Yedjou, C. G., Patlolla, A. K. & Sutton, D. J. (2012). Heavy metal toxicity and the environment. Exp. Suppl., 101, 133-64. https://doi.org/10.1007/978-3-7643-8340-4_6
van der Meer, J. R. & Belkin, S. (2010). Where microbiology meets microengineering: design and applications of reporter bacteria. Nat. Rev. Microbiol., 8(7), 511-22. https://doi.org/10.1038/nrmicro2392
Vega-Corrales, L. & Marín-Vindas C. (2021). Effect of metal concentration on growth and luminescence of luminous bacteria strains isolated from golfo de Nicoya, Costa Rica. Rev. Mar. Cost., 13(1), 27-38. http://dx.doi.org/10.15359/revmar.13-1.2
Vishnivetskaya, T. A., Mosher, J. J., Palumbo, A. V., Yang, Z. K., Podar, M., Brown, S. D., Brooks, S. C., ... & Elias, D. A. (2011). Mercury and other heavy metals influence bacterial community structure in contaminated tennessee streams. Appl. Environ. Microbiol., 77(1), 302-311. http://dx.doi.org/10.1128/AEM.01715-10
Wang, Z., Cong, T. D., Zhong, W., Lau, J. W., Kwek, G., … & Xing, B. (2021). Cyanine-Dyad Molecular Probe for the Simultaneous Profiling of the Evolution of Multiple Radical Species During Bacterial Infections. Angew. Chem. Int. Ed., 60, 16900-16905. https://doi.org/10.1002/anie.202104100
Westrich, J. R. (2015). Consilience of iron in the ecology of vibrio bacteria. (Tesis de Doctorado no publicada), Universidad de Creighton, Georgia.
Wie, M.-A., Oh, S.-J., Kim, S.-C., Kim, R.-Y., Lee, S.-P., Kim, W.-I. & Yang, J.-E. (2012). Toxicity assessment of silver ions compared to silver nanoparticles in aqueous solutions and soils using microtox bioassay. Korean J. Soil Sci. Fert., 45(6), 1114-1119. http://dx.doi.org/10.7745/KJSSF.2012.45.6.1114
Yang, J., Hu, S., Liao, A., Weng, Y., Liang, S. & Ling., Y. (2022). Preparation of freeze-dried bioluminescent bacteria and their application in the detection of acute toxicity of bisphenol A and heavy metals. Food Sci. Nutr., 10(6), 1841-1853. https://doi.org/10.1002/fsn3.2800
Yang, M., Li, J. & Wu, H. (2023). Toxicity evaluation of chlorinated natural water using Photobacterium phosphoreum: Implications for ballast water management. J. Environ. Manage., (335), 11747. https://doi.org/10.1016/j.jenvman.2023.117471
Zarubina, A. P., Deev, L. I., Parkhomenko, I. M., Parshina, E. Y., Sarycheva, A. S., Novoselova, L. A., Lukashev, E. P., … & Rubin, A. B. (2015). Evaluation of toxicity of silver ions and nanoparticles using model bacteria with luminescent phenotype. Nanotechnol. Russi., 10, 475-483. https://doi.org/10.1134/S1995078015030209
Descargas
Publicado
Cómo citar
Número
Sección
Licencia
Esta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial-CompartirIgual 4.0.
Condiciones generales
Revista Ciencias Marinas y Costeras por Universidad Nacional se encuentra bajo una Licencia Creative Commons Atribución-NoComercial-SinDerivadas 3.0 Costa Rica.
La revista se aloja en repositorios de acceso abierto como el Repositorio Institucional de la Universidad Nacional, el Repositorio Kimuk de Costa Rica y la Referencia.
La fuente editorial de la revista debe reconocerse. Para ello utilice el identificador doi de la publicación.
Política de autoarchivo: La revista permite el auto archivo de los artículos en su versión arbitrada, editada y aprobada por el Consejo Editorial de la Revista para que sean disponibles en Acceso Abierto a través de Internet. Más información en el siguiente link: https://v2.sherpa.ac.uk/id/publication/28915
Los artículos de la Revista Ciencias Marinas y Costeras se encuentra bajo la 
