Bioprospecting study, antibiotic and antioxidant activity of the santol’s fruit (Sandoricum koetjape)
DOI:
https://doi.org/10.15359/ru.33-1.6Keywords:
organic chemistry, phytochemistry, antibiotic, antioxidant, Folin-Ciocalteu, DMAC, Kirby-BauerAbstract
A bioprospection was conducted of santol fruit exocarp (Sandoricum koetjape), in order to evaluate its main antioxidant and antibiotic properties. In addition, total polyphenols and proanthocyanidins present were characterized. In order to obtain pure metabolites or more concentrated blends, dry and ground exocarp was subjected to different quantitative tests and chromatographic fractions with various resins. Fractions and extracts were analyzed using the Folin-Ciocalteu (total polyphenols), DMAC (total proanthocyanidins) and DPPH (antioxidant activity) method, using a traditional pattern and one from the sample. Results obtained with the latter were up to 80% higher compared to traditional patterns. It was also determined that fractions with a higher proanthocyanidin content have a higher antibiotic activity, that is 73 ± 4% on the S. Aureus battery and 42 ± 2% on Bacilus spp., both analyzed using the Kirby-Bauer method.
References
Carmona, Y., García, M. V., Igartuburu, J. M., & Barroso, C. G. (2014). Simplification of the DPPH assay for estimating the antioxidant activity of wine and wine by-products. Food chemistry, 165, 198-204. doi: https://doi.org/10.1016/j.foodchem.2014.05.106
Cunningham, D., Vannozzi, S., Turk, R., y Roderick, R. (2005). Constituyentes fitoquímicos del arándano americano (Vaccinium macrocarpon) y sus beneficios para la salud. Revista de fitoterapia, 5-16.
González, I. M. y Serrano, E. A. (2011). Modelo de desarrollo económico local basado en alternativas de uso de plantas medicinales con componentes bioactivos. Biocenosis, 25(1-2).
Hernández, H. D. (2015). Effect of a program of institutional physical activity on oxidative stress in the elderly with and without metabolic syndrome/Efecto de un programa de actividad física institucional sobre el estrés oxidativo en el adulto mayor con y sin síndrome metabólico. International Journal of Integrated Care, 15(8). doi: https://doi.org/10.5334/ijic.2312
Hudzicki, J. (2009). Kirby-Bauer disk diffusion susceptibility test protocol. American society for microbiology, 15(55-63).
Prior, R.L., Fan, E., Hongping, J., Howeel, A., Payne, M. & Reed, J. (2010). Multi-laboratory validation of a standard method for quantifying proanthocyanidins in Cranberry powders. L Sci food Agri; 90; USA, 1473-1478. doi: https://doi.org/10.1002/jsfa.3966
Rodríguez C., E., Gaitán I., R., Méndez C., D., Martelo, J., & Zambrano, R. (2007). Análogos de quinonas naturales con actividad antibacteriana. Scientia et technica, 1(33). doi:http://dx.doi.org/10.22517/23447214.6051
Sáenz, M. R., Acosta, M., Muiser, J., & Bermúdez, J. L. (2011). Sistema de salud de Costa Rica. Salud Pública de México, 53(Supl. 2), s156-s167. Recuperado de http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0036-36342011000800011&lng=es&tlng=es
Sánchez, J. C., Benavides, J., Heredia, J., Cisneros, L., & Jacobo, D. (2013). The Folin-Ciocalteu assay revisited: improvement of its specificity for total phenolic content determination. Analytical Methods, 5(21), 5990-5999. doi: https://doi.org/10.1039/c3ay41125g
Vázquez, A. A., Álvarez, E., López, J.A., Wall, A., y De la Rosa L. A. (2012). Taninos hidrolizables y condensados: naturaleza química, ventajas y desventajas de su consumo. Tecnociencia Chihuahua. Vol. VI, N. º 2, mayo-agosto, 84-93
Villanueva, J. E., Condezo, L. A., y Ramírez, E. (2010). Antocianinas, ácido ascórbico, polifenoles totales y actividad antioxidante, de la cascara de camu-camu. (Myrciaria dubia (H. B. K.) Mc Vaugh). Cienc, Tecnol. Aliment, Campinas, 30(1), 151-160. doi https://doi.org/10.1590/S0101-20612010000500023
Wallace, T., & Giusti, M. (2010). Evaluation of Parameters that Affect the 4-Dimethylaminocinnamaldehyde Assay for Flavanols and Proanthocyanidins. Journal of Food Science, 75: 619-625. doi: https://doi.org/10.1111/j.1750-3841.2010.01734.x
Youngson, R. (2003). Antioxidantes y radicales libres. Madrid: Edaf.
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