Bacterial pathogens and their antimicrobial resistance in tilapia culture in Guatemala
DOI:
https://doi.org/10.15359/ru.35-2.4Keywords:
Aquaculture, antibiotics, antimicrobial, bacteria, OreochromisAbstract
The aim of this study was to identify the main bacterial pathogens affecting tilapia aquaculture in Guatemala during 2018-2019 and evaluate their resistance to the most widely used antibiotics in the aquaculture industry of the area. Fish weighing between 50 and 1,000 g were collected from different production centers. The most frequent external and internal macroscopic signs were recorded for each organism. In addition, bacteriological samples of blood fluid, ulcers and other hemorrhagic areas, anterior kidney, spleen, meninges, liver, and heart were isolated. The isolated pathogenic bacteria were identified at the species level through biochemical tests. Finally, susceptibility was assessed with the following antibiotics oxytetracycline (40 µg), florfenicol (40 µg), enrofloxacin (40 µg), and fosfomycin (40 µg) by the disk diffusion method. The most common clinical signals were skin ulcers, hyperemic areas, body melanization, pale and soft liver, liver vasculitis, and intestinal congestion and bleeding. A total of 16 pathogenic bacteria strains were isolated from tilapia samples. The most frequent genera were Aeromonas (70%: 3 spp), followed by Plesiomonas, Burkholderia, Pseudomonas, Shewanella, and Streptococcus (6%; 1 sp). Isolated bacteria strains showed resistance to oxytetracycline (25%) and fosfomycin (25%) and sensitivity to florfenicol and enrofloxacin. This is the first report related to the most frequent bacterial pathogens and their antimicrobial resistance in tilapia culture in Guatemala.
References
Abdel-Tawwab, M., Ahmad, M. H., Seden, M. E., & Sakr, S. F. M. (2010). Use of Green Tea, Camellia sinensis L., in practical diet for growth and protection of Nile tilapia, Oreochromis niloticus (L.), against Aeromonas hydrophila infection. Journal of the World Aquaculture Society, 41(2), 203–213. https://doi.org/10.1111/j.1749-7345.2010.00360.x
Aisyhah, M. A. S., Amai, M. N. A., Zamri-Saad, M., Siti-Zahrah, A., & Shaquinah, N. N. (2014). Streptococcus agalactiae isolates from cultured fishes in Malaysia manifesting low resistance pattern towards selected antibiotics. Journal of Fish Diseases, 38(12), 1093–1098. https://doi.org/10.1111/jfd.12351
Akinbowale, O. L., Peng, H., & Barton, M. D. (2006). Antimicrobial resistance in bacteria isolated from aquaculture sources in Australia. Journal Applied Microbiology, 100(5), 1103–1113. https://doi.org/10.1111/j.1365-2672.2006.02812.x
Alderman, D., & Smith, P. (2001). Development of draft protocols of standard reference methods for antimicrobial agent susceptibility testing of bacteria associated with fish diseases. Aquaculture, 196(3-4), 211–243. https://doi.org/10.1016/S0044-8486(01)00535-X
Anshary, H., Kurniawan, R. A., Sirwualan, S., Ramli, R., & Baxa, D. V. (2014). Isolation and molecular identification of the etiological agents of streptococcosis in Nile tilapia (Oreochromis niloticus) cultured in net cages in Lake Sentani, Papua, Indonesia. SpringerPlus, 3(627), 1–11. https://doi.org/10.1186/2193-1801-3-627
Ashiru, A. W., Uaboi-Egbeni, P. O., Oguntowo, J. E., & Idika, C. N. (2011). Isolation and antibiotic profile of Aeromonas species from Tilapia fish (Tilapia nilotica) and Catfish (Clarias betrachus). Pakistan Journal Nutrition, 10(10), 982–986. https://10.3923/pjn.2011.982.986
Austin, B., & Austin, D. A. (2012). Bacterial Fish Pathogens. Diseases of Farmed and Wild Fish (5th ed.) Springer.
Brown, J. H. (1989). Antibiotics: their use and abuse in aquaculture. World Aquaculture, 20(2), 34 - 43.
Buller, N. B. (2004). Bacteria from fish and other aquatic animals: A practical identification manual. CABI, Publishing.
Chitmanat, C., Lebel, P., Whangchai, N., Promya, J., & Lebel, L. (2016). Tilapia diseases and management in river-based cage aquaculture in northern Thailand. Journal of Applied Aquaculture, 28(1), 9–16. https://doi.org/10.1080/10454438.2015.1104950
Dong, H. T., Nguyen, V. V., Le, H. D., Sangsuriya, P., Jitrakorn, S.; Saksmerprome, V., Senapin, S., & Rodkhum, C. (2015). Naturally concurrent infections of bacterial and viral pathogens in disease outbreaks in cultured Nile tilapia (Oreochromis niloticus) farms. Aquaculture, 448(1), 427–435. https://doi.org/10.1016/j.aquaculture.2015.06.027
Dong, H. T., Techatanakitarnan, C., Jindakittikul, P., Thaiprayoon, A., Taengphu, S., Charoensapsri, W., Khunrae, P., Rattanarojpong, T., & Senapin, S. (2017). Aeromonas jandaei and Aeromonas veronii caused disease and mortality in Nile tilapia, Oreochromis niloticus (L.). Journal of Fish Diseases, 40(10), 1395–1403. https://doi.org/10.1111/jfd.12617
Espinosa, A., & Bermúdez, M. C. (2012). La acuicultura y su impacto al medio ambiente. Centro de Investigaciones para el Desarrollo, 2, 219–232.
FAO. (2015). The state of world fisheries and aquaculture (SOFIA) 2015. [En línea]: Disponible en http://www.fao.org/3/a-i5555e.pdf
FAO. (2018). FishStat fishery statistical collections: Aquaculture production (2017). [En línea]: http://www.fao.org/fhishery/statitics/software/fishstat/
Figueiredo, H. C. P., Nobrega-Netto, L., Leal, C. A. G., Pereira, U. P., Mian, G. F. (2012). Streptococcus iniae outbreaks in Brazilian Nile tilapia (Oreochromis niloticus, L) farms. Brazilian Journal Microbiology, 43(2), 576–580. http://dx.doi.org/10.1590/S1517-83822012000200019
Fitzsimmons, K. (2013). The success story of the Tilapia industry; current and future trends of the US seafood market. [En línea]: https://www.ctahr.hawaii.edu/sustainag/workshop/downloads/tilapia/3_Weidenbach.pdf
Hammer, O., Harper, D. A., & Ryan, P. D. (2004). PAST, Paleontological statistics version 2.08 [En línea] http://folk.uio.no/ohammer/past
Hernández, E., Figueroa, J., & Iregui C. (2009). Streptococcosis on a red tilapia, Oreochromis sp., farm: a case study. Journal of Fish Diseases, 32(3), 247–252.https://doi.org/10.1111/j.1365-2761.2008.00981.x
Igbinosa, I. H., Beshiru, A., Odjadjare, E. E., Ateba, C. N., & Igbinosa, E. O. (2017). Pathogenic potentials of Aeromonas species isolated from aquaculture and abattoir environments. Microbial Pathogenesis, 107, 185–192. https://doi.org/10.1016/j.micpath.2017.03.037
Jacobs, L., & Chenia, H. Y. (2007). Characterization of integrons and tetracycline resistance determinants in Aeromonas spp. isolated from South African aquaculture systems. International Journal of Food Microbiology, 114(3), 295–306. https://doi.org/ 10.1016/j.ijfoodmicro.2006.09.030.
Li, Y., & Cai, S. H. (2011). Identification and pathogenicity of Aeromonas sobria on tail-rot disease in juvenile tilapia Oreochromis niloticus. Current Microbiology, 62(2), 623–627. https://doi.org/10.1007/s00284-010-9753-8
Liao, C. H., & Shollenberger, L. M. (2003). Survivability and long-term preservation of bacteria in water and in phosphate-buffered saline. Letters in Applied Microbiology, 37(1), 45–50. https://doi.org/10.1046/j.1472-765X.2003.01345.x
Marroquín-Mora, C., & García-Pérez, J. (2015). Evaluación in vitro de extractos de plantas medicinales y probióticos como posibles agentes antimicrobianos para el control de las infecciones bacterianas más comunes en tilapia Oreochromis spp. Universidad San Carlos de Guatemala, Dirección General de Investigación.
Meyburgh, C. M., Bragg, R. R., & Boucher, C. E. (2017). Lactococcus garvieae: an emerging bacterial pathogen of fish. Diseases of Aquatic Organisms, 123, 67–79. https://doi.org/10.3354/dao03083
Mo., W. Y., Chen, Z., Leung, H. M., & Leung, A. O. W. (2017). Application of veterinary antibiotics in China´s aquaculture industry and their potential human health risks. Environmental Science and Pollution Research, 24, 8978-8989. https://doi.org/10.1007/s11356-015-5607-z
Monteiro, S. H., Garcia, F., Gozi, K. S., Romera, D. M., Francisco, J. G., Moura-Andrade, G. C. R., & Tornisielo, V. L. (2016). Relationship between antibiotic residues and occurrence of resistant bacteria in Nile tilapia (Oreochromis niloticus) cultured in cage-farm. Journal of Environmental Science and Health, Part B, 51(12), 817–823. https://10.1080/03601234.2016.1208457
Mzula, A., Wambura, P. N., Mdegela, R. H., & Shirima, G. M. (2020). Present status of aquaculture and the challenge of bacterial diseases in freshwater farmed fish in Tanzania; A call for sustainable strategies. Aquaculture and Fisheries (In press). https:// doi.org/10.1016/j.aaf.2020.05.003
Noga, E. J. (2010). Fish disease: Diagnosis and treatment. Iowa: John Wiley and Sons.
Organización Mundial de Sanidad Animal. (2013). Código sanitario para los animales acuáticos. ISBN 978-92-9044-918-8. [En línea]: https://www.oie.int/doc/ged/D12819.PDF
Plumb, J. A., & Hanson, L. A. (2010). Health Maintenance and Principal Microbial Diseases of Cultured Fishes. Iowa: Wiley-Blackwell.
Raj, N. S., Swaminathan, T. R., Dharmaratnam, D. A., Raja, S. A., Ramraj, D., & Lal, K. K. (2019). Aeromonas veronii caused bilateral exophthalmia and mass mortality in cultured Nile tilapia, Oreochromis niloticus (L.) in India. Aquaculture, 512(13), 1–8. https://10.1016/j.aquaculture.2019.734278
Shayo, S. D., Mwita, C. J., & Hosea, K. M. (2012). Virulence of Pseudomonas and Aeromonas bacteria recovered from Oreochromis niloticus (Perege) from Mtera hydropower Dam; Tanzania. Annals of Biological Research, 3(11), 5157–5161.
Shuxia, L., & Levin, R. E. (2010). Shewanella in a tilapia fish farm. Journal of Fisheries Sciences, 4(2), 159–170. https://10.3153/jfscom.2010016
Sierralta-Chichizola, V., Mayta-Huatuco, E., & León-Quispe, J. (2016). Primer registro de Plesiomonas shigelloides como patógeno oportunista de tilapia Oreochromis niloticus (Linnaeus, 1758) en una Piscigranja de Lima, Perú. Revista de Investigaciones Veterinarias del Perú, 27(3), 565–572. http://dx.doi.org/10.15381/rivep.v27i3.11996.
Stratev, D., & Odeyemi. O. A. (2016). Antimicrobial resistance of Aeromonas hydrophila isolated from different food sources: A mini-review. Journal of Infection and Public Health, 9(5), 535–544. http://dx.doi.org/10.1016/j.jiph.2015.10.006
Zhigang, L., Ke, X.; Lu, M., Gao, F., Cao, J., Zhu, H., & Wang, M. (2015). Identification and pathological observation of a pathogenic Plesiomonas shigelloides strain isolated from cultured tilapia (Oreocrhomis niloticus). Acta Microbiologica SINICA, 55(1), 96–106.
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