Distribution, richness, and molecular identification of spider crabs (Brachyura: Majoidea: Mithracidae) from Costa Rica
DOI:
https://doi.org/10.15359/ru.36-1.18Keywords:
mtDNA, decapods, phylogenetics, molecular identification, taxonomic placementAbstract
The objective of this study was to determine the distribution, richness, and molecular identification of spider crabs in the Costa Rican Pacific and Caribbean coasts. Distribution maps were created for species of the Mithracidae family on the Caribbean and Pacific coasts of Costa Rica, based on exploratory samplings and spatio-temporal records of collections (1960 to 2016) provided by the Universidad de Costa Rica’s Museum of Zoology. In addition, phylogenetic relationships were explored using a multigene concatenated phylogenetic tree based on mitochondrial and nuclear gene fragments. Historical records reveal the presence of 24 species of mithracid crabs and their geographic distribution in the Pacific and Caribbean of Costa Rica, including islands on the continental and oceanic shelf. Molecular analyses confirm sister relationships between the clades Ala-Maguimithrax, Omalacantha-Microphrys, Omalacantha+Microphrys-Teleophrys, and a well-supported clade of species belonging to the genus Mithraculus. However, our concatenated molecular data set suggests a monophyletic relationship between Thoe, Petramithrax, and Hemus, differing from what was previously reported. The results obtained in this study will help in the development of strategies for the environmental protection of these marine resources, by providing a baseline for monitoring environmental degradation and destruction of habitats used by spider crabs on the coasts of Costa Rica. Furthermore, the molecular information obtained in this study will help future studies focusing on understanding the population genetics, phylogeography, regional gene flow, and divergence among species between the Caribbean and Pacific coast.
References
Alvarado, J. J., & Vargas-Castillo, R. (2012). Invertebrados asociados al coral constructor de arrecifes Pocillopora damicornis en Playa Blanca, Bahía Culebra, Costa Rica. Rev Biol Trop, 60, 77-92. https://doi.org/10.15517/RBT.V60I2.19965
Alves, D. F., Barros-Alves, S.D., Cobo, V. J., Lima, D. J., & Fransozo, A. (2012). Checklist of the brachyuran crabs (Crustacea: Decapoda) in the rocky subtidal of Vitória Archipelago, southeast coast of Brazil. Check List, 8(5), 940-950. https://doi.org/10.15560/8.5.940
Assugeni, C. O., Magalhães, T., Bolaños, J. A., Tudge, C. C., Mantelatto, F. L., & Zara, F. J. (2017). Ultrastructure of spermatozoa of spider crabs, family Mithracidae (Crustacea, Decapoda, Brachyura): Integrative analyses based on morphological and molecular data. J. Morphol, 278(12), 1628-1646. https://doi.org/10.1002/jmor.20737
Baeza, J. A., Holstein, D., Umaña-Castro, R., & Mejía-Ortíz, L. M. (2019). Population genetics and biophysical modeling inform metapopulation connectivity of the Caribbean king crab Maguimithrax spinosissimus. Mar Ecol Prog Ser, 610, 83-97. https://doi.org/10.3354/meps12842
Baeza, J. A., Bolaños, J. A., Fuentes, S., Hernandez, J. E., Lira, C., & López, R. (2010). Molecular phylogeny of enigmatic Caribbean spider crabs from the Mithrax-Mithraculus species complex (Brachyura: Majidae: Mithracinae): ecological diversity and a formal test of genera monophyly. J Mar Biol Assoc UK, 90(4), 851-858. https://doi.org/10.1017/S0025315409991044
Bell, T. (1836). Some account of the Crustacea of the coasts of South America, with descriptions of new genera and species; founded principally on the collections obtained by Mr Cuming and Mr Miller. The Transactions of the Zoological Society of London, 2, 1-86. https://doi.org/10.1111/j.1469-7998.1839.tb00007.x
Bucklin, A., Steinke, D., & Blanco-Bercial, L. (2011). DNA barcoding of marine metazoa. Ann Rev Mar Sci, 3, 471‑508. https://doi.org/10.1146/annurev-marine-120308-080950
Bucklin, A., Lindeque, P., Rodríguez-Ezpeleta, N., Albaina, A., & Lehtiniemi, M. (2016). Metabarcoding of marine zooplankton: prospects, progress, and pitfalls. J Plankton Res, (38), 393‑400. https://doi.org/10.1093/plankt/fbw023
Carmona-Suárez, C., & Poupin, J. (2016). Majoidea crabs from Guadeloupe Island, with a documented list of species for the Lesser Antilles (Crustacea, Decapoda, Brachyura, Majoidea). Zoosystema, 38(3), 353-387. https://doi.org/10.5252/z2016n3a5
Castresana, J. (2000). Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Mol. Biol. Evol, 17(4), 540-552. https://doi.org/10.1093/oxfordjournals.molbev.a026334
Colavite, J., Windsor, A. M., & Santana, W. (2020). A new genus for Pericera septemspinosa Stimpson, 1871 and Pericera heptacantha Bell, 1836 (Crustacea, Brachyura, Majoidea), based on morphology and molecular data. Zoosyst Evol, 96(1), 205-216. https://doi.org/10.3897/zse.96.50360
Colgan, D., McLauchlan, A., Wilson, G. D., Livingston, S., Edgecombe, G. D., Macaranas, J., Cassis, G., & Gray, M. R. (1998). Histone H3 and U2 snRNA DNA sequences and arthropod molecular evolution. Aust. J. Zoo., 46(5), 419-437. https://doi.org/10.1071/ZO98048
Cruz, C. N., & Campos, N.H. (2003). Los cangrejos Araña (Decapoda: Bracyura: Majoidea) del Caribe colombiano. Biota Colomb, 4(2), 261-269. http://revistas.humboldt.org.co/index.php/biota/article/view/136
De Grave, S., Pentcheff, N. D., Ahyong, S. T., Chan, T. Y., Crandall, K. A., Dworscha, P. C., Felder, D. L., Feldmann, R. M., Fransen, C. H .J. M., Goulding, L. Y. D., Lemaitre, R., Low, M. E. Y., Martin, J. W., Ng, P. K. L., Schweitzer, C. E., Tan, S. H., Tshudy, D., & Wetzer, R. (2009). A classification of living and fossil genera of decapod crustaceans. Raffles Bull. Zool, 21, 1-109.
De Saussure, H. (1853). Description de quelques crustacés nouveaux de la côte occidentale du Mexique. Revue et Magasin de Zoologie pure et appliquée, 2(5), 354-368.
Desbonne, I., & Schramm, A. (1867). Brachyures. In ‘Crustacés de la Guadeloupe d’aprés un Manuscrit du Docteur Isis Desbonne comparé avec les Échantillons de Crustacés de sa Collection et les Derniéres Publications de M.M. Henri de Saussure et William Stimpson. Premiere partie. Nabu Press. https://doi.org/10.5962/bhl.title.10402
Folmer, O., Black, M., Hoeh, W., Lutz, R., & Vrijenhoek, R. (1994). DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotechnol, 3(5), 294-299.
García, Y. L. D., & Capote, A.J. (2015). List of marine crabs (Decapoda: Anomura and Brachyura) of shallow littoral of Santiago de Cuba, Cuba. Check List, 11(2), 1-22. https://doi.org/10.15560/11.2.1601
Garth, J. S. (1940). Some new species of brachyuran crabs from Mexico and the Central and South American mainland. Allan Hancock Pacific Expeditions, 5, 53-127.
Geiger, M., Moriniere, J., Hausmann, A., Haszprunar, G., Wägele, W., Hebert, P., & Rulik, B. (2016). Testing the Global Malaise Trap Program How well does the current barcode reference library identify flying insects in Germany? Biodivers Data J, 4, e10671. https://doi.org/10.3897/bdj.4.e10671
Gerstaecker, A. (1857). Carcinologishes beiträge. Archiv für Naturgeschichte, 22, 101-162.
González-Gómez, R., Briones-Fourzán, P., Álvarez-Filip, L., & Lozano-Álvarez, E. (2018). Diversity and abundance of conspicuous macrocrustaceans on coral reefs differing in level of degradation. PeerJ, 6, e4922. https://doi.org/10.7717/peerj.4922
Gutiérrez-Salcedo, J. M., Aguilar-Pérez, M. I., Bermúdez, A., Campos, N. H., & Navas-S, G. R. (2007). Invertebrate macrofaunal structure present in nests of Malacanthus plumieri (Bloch, 1786) (Perciformes: Malacanthidae) in Nenguange Bay, Tayrona Natural Park, Colombian Caribbean sea. Caldasia, 29(2), 309-328.
Herbst, J. F. W. (1782-1804). Versuch einer Naturgeschichte der Krabben und Krebse nebst einer systematischen Beschreibung ihren verschiedenen Arten. Berlin und Stralsund. https://doi.org/10.5962/bhl.title.64679
Hollier, J. (2020). The Decapoda (Crustacea) described by Henri de Saussure. Rev Suisse Zool, 125(2), 291-298. https://doi.org/10.5281/zenodo.1414227
Hultgren, K. M., Guerao, G., Marques, F. P. L., & Palero, F. P. (2009). Assesing the contribution of molecular and larval morphological characters in a combined phylogenetic analysis of the superfamily Majoidea. 437-455. In Martin, J. W.; Crandall, K. A.; & Felder, D. L. (Eds.), Decapod Crustacean Phylogenetics. CRC Press, Taylor & Francis Group.
Hultgren, K. M., & Stachowicz, J. J. (2008). Molecular phylogeny of the brachyuran crab superfamily Majoidea indicates close congruence with larval morphology-based trees. Mol Phylogenet Evol, 48(3), 986-996. https://doi.org/10.1016/j.ympev.2008.05.004
Kalyaanamoorthy, S., Minh, B. Q., Wong, T. K., von Haeseler, A., & Jermiin, L. S. (2017). ModelFinder: fast model selection for accurate phylogenetic estimates. Nat. Methods, 14, 587-589. https://doi.org/10.1038/nmeth.4285
Katoh, K., & Standley, D. M. (2013). MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol. Biol. Evol, 30(4), 772-780. http://dx.doi.org/10.1093/molbev/mst010
Klompmaker, A. A., Portell, R. W., Klier, A. T., Prueter, V., & Tucker, A. L. (2015). Spider crabs of the Western Atlantic with special reference to fossil and some modern Mithracidae. Peer J, 3, e1301. https://doi.org/10.7717/peerj.1301
Lamarck, J. B. P. A. (1818). Histoire naturelle des animaux sans vertèbres, présentant les caractères généraux et particuliers de ces animaux, leur distribution, leurs classes, leurs familles, leurs genres et la citation des principales espèces qui s’y rapportent; précédé d’une introduction offrant la détermination des caractères essentiels de l’animal, sa distinction du végétal et des autres corps naturels, enfin l’exposition des principes fondamentaux de la zoologie. Verdière. https://doi.org/10.5962/bhl.title.63986
Mantelatto, F. L., Tamburus, A. F., Magalhães, T., Buranelli, R.C., Terossi, M., Negri, M., Castilho, A. L., Costa, R. C., & Zara, F. J. (2020). Checklist of decapod crustaceans from the coast of the São Paulo state (Brazil) supported by integrative molecular and morphological data: III. Infraorder Brachyura Latreille, 1802. Zootaxa, 4872(1), 1-108.
Martin, J. W., and Davis, G. E. (2006). Historical trends in crustacean systematics. Crustaceana., 79, 1347-1368. https://doi.org/10.1163/156854006779277321
Milne-Edwards, A. (1875). Études sur les Xiphosures et les Crustacés podopthalmaires. In ‘Mission scientifique au Mexique et dans l’Amerique centrale. Recherches Zoologiques a l’histoire de la faune de l’Amerique Centrale et du Mexique. Imprimerie Nationale. https://doi.org/10.5962/bhl.title.119116
Minh, B. Q., Nguyen, M. A. T., & von Haeseler, A. (2013). Ultrafast approximation for phylogenetic bootstrap. Mol Biol Evol, 30(5), 1188-1195. https://doi.org/10.1093/molbev/mst024
Mokady, O., Rozenblatt, S., Graur, D., & Loya, Y. (1994). Coral-host specificity of Red Sea Lithophaga bivalves: interspecific and intraspecific variation in 12S mitochondrial ribosomal RNA. Mol Mar Biol Biotechnol, 3(3), 158-164. https://pubmed.ncbi.nlm.nih.gov/7522832/
Moran, D. A., & Dittel, A. I. (1993). Anomuran and Brachyuran crabs of Costa Rica: annotated list of species. Rev Biol Trop, 41(3), 599-617. https://revistas.ucr.ac.cr/index.php/rbt/article/view/23978
Moscoso, V. (2012). Catálogo de crustáceos decápodos y estomatópodos del Perú. Bol Inst Mar Perú, 27(1-2), 8-207.http://biblioimarpe.imarpe.gob.pe/handle/123456789/2190
Ng, P. K., Guinot, D., & Davie, P. J. (2008). Systema Brachyurorum: Part I. An annotated checklist of extant brachyuran crabs of the world. Raffles Bull Zool, 17, 1-286.
Ng, P. K., Ho, P. H., Lin, C. W., & Yang, C. H. (2018). The first record of an eastern Pacific invasive crab in Taiwanese waters: Amphithrax armatus (Saussure, 1853) (Brachyura: Majoidea: Mithracidae), with notes on the taxonomy of the genus. J Crustac Biol, 38(2), 198-205. https://doi.org/10.1093/jcbiol/rux109
Nguyen, L. T., Schmidt, H. A., von Haeseler, A., & Minh, B.Q. (2015). IQ-TREE: A fast and effective stochastic algorithm for estimating maximum likelihood phylogenies. Mol Biol Evol, 32(1), 268-274. https://doi.org/10.1093/molbev/msu300
R Core Team. (2017). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. https://www.R-project.org/.
Rambaut, A. (2010). FigTree v1.4. Institute of Evolutionary Biology, University of Edinburgh, Edinburgh. http://tree.bio.ed.ac.uk/software/figtree/
Rathbun, M. J. (1892). Catalogue of the crabs of the family Periceridae in the U.S. National Museum. Proceedings of the United States National Museum, 15(901), 231-277. https://doi.org/10.5479/si.00963801.16-927.63
Rathbun, M. J. (1920). New species of spider crabs from the Straits of Florida and Caribbean Sea. Proc Biol Soc Wash, 33, 23-24.
Rhyne, A. L., Fujita, Y., & Calado, R. (2004). Larval development and 1st crab of Mithraculus sculptus (Decapoda: Brachyura: Majoidea: Mithracidae) described from laboratory-reared material. J Mar Biol Assoc UK, 86, 1133-1147. https://doi.org/10.1017/S0025315406014111
Schmidt de Melo, G. A. (1990). Descrição de Mithrax (Mithrax) besnardi, sp.n., espécie abissalbẽntica de ãguas meridionais da América do Sul (Custacea, Brachyura, Majidae). Atlântica, Rio Grande, 12(1), 19-26.
Števcic, Z. (1994). Contribution to the re-classification of the family Majidae. Period Biol, 96, 419-420.
Stimpson, W. (1860). Notes on North American Crustacea, in the Museum of the Smithsonian Institution. Ann N Y Acad Sci, 7(1), 177-246.
Stimpson, W. (1871). Preliminary report on the Crustacea dredged in the Gulf Stream in the Straits of Florida by L. F. de Pourtalés, Assist. United States Coast Survey. Part I. Brachyura. Bull Mus Comp Zool, 2(2), 109-160.
Streets, T. H. (1871). Catalogue of Crustacea from the Isthmus of Panamá. Collected by J. A. McNeil. Proc Acad Nat Sci Phila, 23, 238-243.
Toon, A., Finley, M., Staples, J., & Crandall, K. A. (2009). Decapod phylogenetics and molecular evolution. 17-35. In Martin, J. W.; Crandall, K.A.; & Felder, D. L. (Eds.), Decapod crustacean phylogenetics. Crustacean Issues. CRC Press.
Trifinopoulos, J., Nguyen, L. T., von Haeseler, A., & Minh, B. Q. (2016). W-IQ-TREE: a fast online phylogenetic tool for maximum likelihood analysis. Nucleic Acids Res, 44(1), 232-235. https://doi.org/10.1093/nar/gkw256
Türkay, M. (1986). Mithrax caboverdianus n.sp., eine neue Seespinnen-Art von den Kapverdischen Inseln (Crustacea: Decapoda: Brachyura: Majidae). Cour. Forsch Inst. Senckenberg, 81,7-11.
Vaidya, G., Lohman, D. J., & Meier, R. (2011). Sequence Matrix: concatenation software for the fast assembly of multi‐gene datasets with character set and codon information. Cladistics, 27(2), 171-180. https://doi.org/10.1111/j.1096-0031.2010.00329.x
Vargas, C. R. (2012). Nuevas adiciones a la fauna de crustáceos decápodos de Bahía Culebra, Guanacaste, Costa Rica. Rev Biol Trop, 60(2), 73-76. https://doi.org/10.15517/RBT.V60I2.19963
Vargas-Castillo, R., & Vargas-Zamora, J. A. (2020). Crustaceans (Decapoda & Stomatopoda) from Golfo Dulce (Pacific, Costa Rica) in the collection of the Museum of Zoology, University of Costa Rica. Cuad Inv UNED, 12(1), 229-244. https://doi.org/10.22458/urj.v12i1.2736
Wagner, H. P. (1990). The genera Mithrax Latreille, 1818 and Mithraculus White, 1847 (Crustacea: Brachyura: Majidae) in the western Atlantic Ocean. Zool Verh, 264(1), 1-65.
Wehrtmann, I. S., & Cortés, J. (2009). Marine Biodiversity of Costa Rica, Central America. Monographiae Biologicae. Springer.
Wickham, H. (2016). ggplot2: Elegant Graphics for Data Analysis. Springer-Verlag New York. ISBN 978-3319-24277-4.https://ggplot2.tidyverse.org.
Windsor, A. M., & Felder, D. L. (2014). Molecular phylogenetics and taxonomic reanalysis of the family Mithracidae MacLey (Decapoda: Bracyura: Majoidea). Invertebr Syst, 28(2), 145-173. https://doi.org/10.1071/IS13011
Windsor, A. M., & Felder, D. L. (2009). Re-evaluation of species allied to Mithrax hispidus (Decapoda: Brachyura: Majoidea: Mithracidae) based on three mitochondrial genes. Zootaxa, 2302(1), 61-68. https://doi.org/10.11646/ZOOTAXA.2302.1.5
Windsor, A. M., & Felder, D.L. (2017). Corrigendum to: Molecular phylogenetics and taxonomic reanalysis of the family Mithracidae MacLeay (Decapoda: Brachyura: Majoidea). Invertebr Syst, 31(2), 232-232. https://doi.org/10.1071/IS13011_CO
WORMS. (2020). World Register of Marine Species. https://www.marinespecies.org/aphia.php?p=taxdetails&id=439056 03/11/2020. https://doi.org/10.14284/170
Downloads
Additional Files
Published
Issue
Section
License
Authors who publish with this journal agree to the following terms:
1. Authors guarantee the journal the right to be the first publication of the work as licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
2. Authors can set separate additional agreements for non-exclusive distribution of the version of the work published in the journal (eg, place it in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
3. The authors have declared to hold all permissions to use the resources they provided in the paper (images, tables, among others) and assume full responsibility for damages to third parties.
4. The opinions expressed in the paper are the exclusive responsibility of the authors and do not necessarily represent the opinion of the editors or the Universidad Nacional.
Uniciencia Journal and all its productions are under Creative Commons Atribución-NoComercial-SinDerivadas 4.0 Unported.
There is neither fee for access nor Article Processing Charge (APC)
