Sensitivity of Hydra attenuata and Hydra viridis to diuron and ethoprophos pesticides, Costa Rica
DOI:
https://doi.org/10.15359/ru.37-1.29Keywords:
Hydra, diuron, ethoprophos, toxicity, pineappleAbstract
[Objective] The objective was to determine which of the two hydra species (Hydra attenuata or Hydra viridis) was more sensitive to two pesticides, and then use the most sensitive species as part of the battery of test organisms of the Ecotoxicological Studies Laboratory (ECOTOX). [Methodology] For this purpose, dilutions of two of the most commonly used pesticides in pineapple cultivation in Costa Rica which were detected in water samples were used: the herbicide diuron and the insecticide ethoprophos. Toxicity bioassays were conducted with each of these substances and with a mixture of these substances. The morphological state of the hydras was recorded. In addition, the mean effective concentration (EC50), mean lethal concentration (LC50) and toxicity interactions were evaluated in terms of three possibilities: antagonistic, additive or synergistic. [Results] H. attenuata was the most sensitive species to both pesticides, with an LC50s of 16.84 mg/L and 103.01 mg/L, and an EC50s of 4.24 mg/L and 23.4 mg/L for diuron and ethoprophos, respectively. Furthermore, H. viridis had an LC50s of 36.94 mg/L and 103.01 mg/L, with an EC50s of 13.64 mg/L and 53.12 mg/L, also for diuron and ethoprophos, respectively. Exposure to the pesticide mixture only caused mortality in H. viridis and had a synergistic effect on both species. [Conclusions] These results suggest that H. attenuata is the most appropriate species for the evaluation of pesticide effects, but further studies should be conducted on the interactions of contaminants in mixtures, since these may modify their toxicity for different species.
References
Andrade, V. (2021). Efectos de la contaminación directa y difusa por plaguicidas sobre el zooplancton a distintas escalas de análisis [tesis de doctorado]. Santa Fe, Argentina: Universidad Nacional del Litoral. https://bibliotecavirtual.unl.edu.ar:8443/bitstream/handle/11185/5764/Tesis.pdf?sequence=1&isAllowed=y
Argüello, E., Chaves, A., Chinchilla, A. y Navarrete, M. (2015). Posibles efectos en la salud asociados a la exposición al agua para consumo humano contaminada por plaguicidas, en las poblaciones de Milano, Luisiana y El Cairo, Siquirres, estudiados en el periodo de setiembre 2013 a enero de 2014 [Seminario de grado]. San José: Universidad de Costa Rica. http://repositorio.sibdi.ucr.ac.cr:8080/jspui/bitstream/123456789/3368/1/39201.pdf
Aronzon, C. (2013). Evaluación de la toxicidad de los contaminantes cobre, nonilfenol y diazinón sobre embriones y larvas de Rhinella (Bufo) arenarum [tesis de posgrado]. Argentina: Universidad de Buenos Aires. https://bibliotecadigital.exactas.uba.ar/download/tesis/tesis_n5340_Aronzon.pdf
Barrera, J., Espinosa, A. y Álvarez, J. (2019). Contaminación en el Lago de Tota, Colombia: Toxicidad aguda en Daphnia magna (Cladocera: Daphniidae) e Hydra attenuata (Hydroida: Hydridae). Revista de Biología Tropical, 67(1), 11-23. http://dx.doi.org/10.15517/rbt.v67i1.33573
Caseley, J.C. (1996). Capítulo 10. Herbicidas. En Labrada, R., Caseley, J.C. y Parker, C. Manejo de malezas para países en desarrollo. FAO.
Castro, S., Picado, H. y Vega, W. (2015). Comunidades fumigadas en Costa Rica acusan al Estado y transnacionales ante la Comisión Interamericana de Derechos Humanos. Revista Biodiversidad, 85, 23-26. https://www.grain.org/media/w1siziisijiwmtuvmduvmduvmtbfnddfmtnfmjq3xza2q29tdw5pzgfkzxnfznvtawdhzgfzx2vux0nvc3rhx1jpy2ffywn1c2fux2fsx0vzdgfkb195x3ryyw5zbmfjaw9uywxlc19hbnrlx2xhx0nvbwlzav9ux0ludgvyyw1lcmljyw5hx2rlx0rlcmvjag9zx0h1bwfub3mucgrmil1d
Córdoba, L. (2015). Evaluación de la contaminación ambiental en aire y polvo por plaguicidas, en 12 centros educativos del cantón de Matina, Limón [tesis de posgrado]. San José: Universidad de Costa Rica. https://www.kerwa.ucr.ac.cr/bitstream/handle/10669/84505/Tesis%20versi%c3%b3n%20final%20200715_license.pdf?sequence=1&isAllowed=y
Dal, J. (2020). Effects of the herbicide atrazine on members of the freshwater genus Hydra. https://jayscholar.etown.edu/cgi/viewcontent.cgi?article=1008&context=biostu
DeLorenzo, M. y Serrano, L. (2007). Individual and Mixture Toxicity of Three Pesticides: Atrazine, Chlorpyrifos, and Chlorothalonil to the Marine Phytoplankton Species Dunaliella tertiolecta. Journal of Environmental Science and Health, 38(5), 529-538. https://doi.org/10.1081/PFC-120023511
Demetrio, P. (2012). Estudio de efectos biológicos de plaguicidas utilizados en cultivos de soja RR y evaluación de impactos adversos en ambientes acuáticos de agroecosistemas de la región pampeana [tesis de doctorado]. Argentina: Universidad Nacional de La Plata. https://doi.org/10.35537/10915/18139
Echeverría-Sáenz, S., Mena, F., Pinnock, M., Ruepert, C., Solano, K., de la Cruz, E., Campos, B., Sánchez-Avila, J., Lacorte, S. y Barata, C. (2012). Environmental hazards of pesticides from pineapple crop production in the Río Jiménez watershed (Caribbean Coast, Costa Rica). Science of the Total Environment. 440, 106-114. https://doi.org/10.1016/j.scitotenv.2012.07.092
Escobar-Chávez, C., Alvariño, L. y Iannacone, I. (2019). Evaluación del riesgo ambiental acuático de la mezcla de los plaguicidas imidacloprid (insecticida) y propineb (fungicida) en Daphnia magna straus, 1820. Paideia XXI, 9(2), 301-332. doi:10.31381/paideia.v9i2.2759
Flores, F., Kaserzon, S., Elisei, G., Ricardo, G. y Negri, AP. (2020). Toxicity thresholds of three insecticides and two fungicides to larvae of the coral Acropora tenuis. PeerJ, 8, e9615. https://doi.org/10.7717/peerj.9615
Fournier, L., Castillo, L., Ramírez, F., Moraga, G. y Ruepert, C. (2019). Preliminary Assessment of Agricultural Area and its Influence to the Quality of Water in Golfo Dulce, Costa Rica. Revista de Ciencias Ambientales, 53(1). http://dx.doi.org/10.15359/rca.53-1.5
Guinou, C. (2010). The Toxicity of Harmony Landfill Leachate to Green Hydra (Hydra viridissima) (tesis de posgrado). Oshawa, Canadá: University of Ontario Institute of Technology. https://ir.library.ontariotechu.ca/xmlui/bitstream/handle/10155/102/Ginou_Carrie.pdf?sequence=2&isAllowed=y
Laboratorios de Referencia de la Unión Europea (EURL). (2019). Analytical quality control and method validation procedures for pesticide residues analysis in food and feed. SANTE/12682/2019. Dirección General de Salud y Seguridad Alimentaria. https://www.eurl-pesticides.eu/userfiles/file/EurlALL/AqcGuidance_SANTE_2019_12682.pdf
Hamada, M., Satoh, N. y Khalturin, K. (2020). A Reference Genome from the Symbiotic Hydrozoan, Hydra viridissima. G3 Genes|Genomes|Genetics, 10(11), 3883-3895. https://doi.org/10.1534/g3.120.401411
Hamada, M., Schröder, K., Bathia, J., Ulrich, K., Fraune, S., Khalturina, M., Khalturin, K., Shinzato, Satoh., N. y Bosch., T. (2018). Metabolic co-dependence drives the evolutionarily ancient Hydra-Chlorella symbiosis. Tancha, Japón: Okinawa Institute of Science and Technology Graduate University. https://elifesciences.org/articles/35122
Hlina, B.L., Birceanu, O., Robinson, C.S., Dhiyebi, H. y Wilkie, M.P. (2021). The relationship between thermal physiology and lampricide sensitivity in larval sea lamprey (Petromyzon marinus). Journal of Great Lakes Research, 47, S272-S284. https://doi.org/10.1016/j.jglr.2021.10.002
Howe, P., Reichelt-Brushett, A., Clark, M. y Seery, C. (2017). Toxicity estimates for diuron and atrazine for the tropical marine cnidarian Exaiptasia pallida and in-hospite Symbiodinium spp. using PAM chlorophyll-a fluorometry. Journal of Photochemistry and Photobiology B: Biology, 171, 125-132. https://doi.org/10.1016/j.jphotobiol.2017.05.006
Instituto Regional de Estudios en Sustancias Tóxicas (IRET-UNA) (2020). Manual de plaguicidas de Centroamérica. http://www.plaguicidasdecentroamerica.una.ac.cr/index.php/base-de-datos-menu
Klimovich, A. y Bosch, T. (2018). Rethinking the Role of the Nervous System: Lessons From the Hydra Holobiont. BioEssays, 40, (1800060), 1-9. https://doi.org/10.1002/bies.201800060
Lima, T. R. R., Martins, A. C., Pereira, L. C. y Aschner, M. (2022). Toxic Effects Induced by Diuron and Its Metabolites in Caenorhabditis elegans. Neurotoxicity Research, 40(6), 1812-1823. https://doi.org/10.1007/s12640-022-00596-2
Mansano, A. S., Moreira, R. A., Dornfeld, H. C., Diniz, L., Daam. M., Rocha, O. y Seleghim, M. (2018). Acute and chronic toxicity of diuron and carbofuran to the neotropical cladoceran Ceriodaphnia silvestrii. Environmental Science and Pollution Research, 25, 13335-13346. https://doi.org/10.1007/s11356-016-8274-9
Murugadas, A., Zeeshan, M., Thamaraiselvi, K., Ghaskadbi, S. & Abdulkader, M. (2016). Hydra as a model organism to decipher the toxic effects of copper oxide nanorod: Eco-toxicogenomics approach. Scientific Reports, 6 (29663), 1-14. https://doi.org/10.1038/srep29663
Obando, A. (2017). El Estado detrás de la piña: El desarrollo de los monocultivos de exportación en la Región Huetar Norte de Costa Rica. 9° Congreso Lationamericano de Ciencia Política. Montevideo: ALACIP. http://www.congresoalacip2017.org/arquivo/downloadpublic2?q=YToyOntzOjY6InBhcmFtcyI7czozNToiYToxOntzOjEwOiJJRF9BUlFVSVZPIjtzOjQ6IjIzNzkiO30iO3M6MToiaCI7czozMjoiNjdhYjNhNzc3ZDgxMDI2M2RjMjQzMzBiYTExOTRkZGUiO30%3D
Olguín, C. (2021). Responses of jellyfish to pesticides across different life history stages (tesis de posgrado). Griffith University. https://doi.org/10.25904/1912/4080
Pesticide Action Network (PAN). (2020). Pesticide info. https://www.pesticideinfo.org/
Ramírez, F. (2017). Diagnóstico de plaguicidas en Caribe y Zona Norte. Heredia, Costa Rica: IRET-UNA.
Tribunal Latinoamericano del Agua (TLA) (2013). Memoria del foro: “El monocultivo de piña en Costa Rica y la repercusión en los sistemas hídricos”. TLA.
Trottier, S., Blaise, C., Kusui, T. y Johnson, E. (1997). Acute toxicity assessment of aqueous samples using a microplate‐based Hydra attenuata assay. Environmental Toxicology and Water Quality, 12(3), 265-271. https://doi.org/10.1002/(sici)1098-2256(1997)12:3<265::aid-tox10>3.0.co;2-9
University of Hertfordshire (2019). PPDB: Pesticide Properties DataBase. http://sitem.herts.ac.uk/aeru/ppdb/en/search.htm
Vargas, C. , Miller, C. y Arguedas, C. (2020). Informe: Monitoreo del estado de la piña en Costa Rica para el año 2018. San José: CONARE, CENAT y Laboratorio PRIAS. https://repositorio.conare.ac.cr/bitstream/handle/20.500.12337/7892/Vargas_C_Informe_monitoreo_pina_CR_ano_2018_2020.pdf?sequence=1&isAllowed=y
Ye, S. (2017). The Roles of Endosymbionts and Hosts in Adaptive Response to Stress. Houston: Rice University. https://hdl.handle.net/1911/94004
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