Diversidad genética entre especies del género lemna (lemnaceae) utilizando fragmentos polimórficos de ADN amplificados al azar (rapds)

Authors

  • Frank Solano Campos Escuela de Ciencias Biológicas, Universidad Nacional.
  • Elizabeth Salas Zúñiga Escuela de Ciencias Biológicas, Universidad Nacional

Keywords:

Genetic similarity, RAPDs, Lemnaceae, AND, Polymorphism.

Abstract

Lemna aequinoctialis and Lemna valdiviana were tested genetic similarity by random amplified polymorphic DNA (RAPD) fingerprinting. Of 60 primers (10-mer) tested, 26 generated polymorphic products. 143 bands were found from 200bp up to 1500bp, 109 were polymorphic (76,2%), with an average of 4,19. Of these, only 18 were specific of L. aequinoctialis, while 91 bands were specific for L. valdiviana. Data were used to generate Jaccard’s similarity coefficients and to construct a dendrogram using UPGMA method in the BiodiversityPro statistical package. It is concluded from this study that there were clear differences (high genetic diversity) between L. aequinoctialis and L. valdiviana and that RAPD sesults are comparable with those obtained from studies on morphology, It is a practical method to assess the relationships between these species.

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Published

2009-01-01

Issue

Vol. 23 No. 1-2 (2009)

Section

Original scientific papers (evaluated by academic peers)

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