Diferenciación molecular de Lemna aequinoctialis y l. Valdiviana (lemnaceae) por patrones proteicos (ING)

Authors

  • Mª del Milagro Montero Rojas
  • Elizabeth Salas Zúñiga
  • Freddy Acuña López

Keywords:

Lemna valdiviana, L. aequinoctialis, total protein, electrophoresis

Abstract

This study was done at the Molecular Genetics Laboratory of Universidad Nacional, Heredia, Costa Rica. Previously identified macrophyte aquatic plants Lemna valdiviana and L. aequinoctialis were used. Usually these species are found in residual water or salt rich water. Recently these species have been considered as indicators of contamination caused by pesticides and herbicides present in the water. Their protein composition achieves levels as high as 38-43% of dry matter, which is important for different practical purposes. Total protein extraction was carried out in reducing conditions, using discontinuous polyacrylamide gel electrophoresis with SDS (SDS-PAGE). Stacking gels were 8 and 10%, resolving gel was 4% and running time was 3-4 hours. Gels were dyed with Coomasie blue. Protein patterns showed genetic differences between both species which might be due to differences in the habitat and in their reproduction type.

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Published

2008-01-01

Issue

Vol. 22 No. 1-2 (2008)

Section

Original scientific papers (evaluated by academic peers)

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