Caracterización molecular por patrones electroforéticos de isoenzimas esterasas en Lemna aequinoctialis y Lemna valdiviana (lemnaceae) de Costa Rica (ING)

Authors

  • Sharon Jiménez Delgado Laboratorio de Genética Molecular, Universidad Nacional, Heredia, Costa Rica, Costa Rica
  • Elizabeth Salas Laboratorio de Genética Molecular, Universidad Nacional, Heredia, Costa Rica, Costa Rica
  • Freddy Acuña López Laboratorio de Genética Molecular, Universidad Nacional, Heredia, Costa Rica, Costa Rica

Keywords:

Lemnaceae, Lemna, isoenzyme, esterases, bioremediation

Abstract

The Lemnaceae family lacks morphologic and anatomical characteristics with comparative aims and for that reason the molecular studies has been necessary a differentiation. In Costa Rica there are two species of Lemna: Lemna aequinoctialis and Lemna valdiviana. With the objective of a molecular characterization and differentiation of both species were extracted isoenzymes esterases. The study was carried out in the Molecular Genetics Laboratory of the School of Biological Sciences at Universidad Nacional, Heredia, Costa Rica. The technical electrophoresis in gel of polyacrylamida SDS-PAGE was used. It was difference in the pattern of isoenzymes between both species. According to previous molecular studies based on morphology and anatomy, flavonoides, isoenzymes, and DNA sequences of certain genes both species are very different; in addition they belong to different monophyletics groups. On the other hand, the importance of this molecular characterization is its possible use in water treatment, because the esterases have had important in the bioremediation. Also, species of the Lemnaceae family are used in the water treatment like bioremediation.

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Published

2008-01-01

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Section

Original scientific papers (evaluated by academic peers)

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