Medroxyprogesterone acetate in homemade intravaginal devices for estrus synchronization in hair ewes: Evaluation of efficacy, adverse effects and comparison with a commercial device in Costa Rican herds.
DOI:
https://doi.org/10.15359/rcv.35-2.3Keywords:
estrus synchronization, ewes, medroxyprogesterone acetateAbstract
The goal of our study was to determine the pharmacological efficacy and adverse effects of a homemade intravaginal device containing medroxyprogesterone acetate for estrus synchronization in hair ewes. A total of 78 adult, multiparous, cycling females were included in the two-phase experiment. During the first phase 18 ewes were included, and the efficacy and adverse effects of the homemade device were evaluated. In the second phase 60 ewes were aleatory assigned into two experimental groups: (G1) homemade intravaginal device (n-30), and (G2) commercial device (n-30) with the goal of comparing the estrus synchronization rate and conception rate among groups when using natural mating. The protocol consisted of 12-days treatment with 60mg of medroxyprogesterone acetate (MAP) by using the intravaginal device, followed by 200 UI eCG and 5 mg dinoprost tromethamine injection on day 12. A total of 13 males were tested by complete andrological evaluation, 8 were included as satisfactory males for the experiments. During both phases no signs of estrus were observed during the treatment with MAP, and 100% showed estrous signs 48 hours after device withdrawal. Adverse effects observed in the first part included: Mild vaginitis in all ewes, 3 females presented adherences, 2 showed fibrinogen increase at day 12, and a general lower leucocyte count after treatment was observed. In the second phase, no differences in estrus synchronization was observed among groups, and conception rates were 80.0% and 76.7% for G1 and G2 respectively. We conclude that the homemade intravaginal device is as effective as a commercial one for estrus synchronization, which allows obtaining high pregnancy rates in hairy sheep in Costa Rica.References
Abecia, J.A., Forcada, F. & González-Bulnes, A. 2012. Hormonal control of reproduction in small ruminants. Anim. Reprod. Sci. 130: 173-179. https://doi.org/10.1016/j.anireprosci.2012.01.011
Bagley, C.V. 1997. Breeding soundness in rams: how to do it and how to interpret it [en línea]. Utah State University Cooperative Extension Service, United States. http://extension.usu.edu/files/publications/factsheet/AH_Sheep_13.pdf
Barrett, D.M.W., Bartlewski, P.M, Batista-Arteaga, M., Syminton, A. & Rawlings, N.C. 2004. Ultrasound and endrocine evaluationof the ovarian response to a single dose of 500 IU of eCG following a 12-days treatment with progesterone-releasing intravaginal sponges in the breeding and nonbreeding season in ewes. Theriogenology 61: 311-327. https://doi.org/10.1016/S0093-691X(03)00215-2
Bidinost, F., Gibbons, A.E. & Cueto, M. 2001. Ecografía para el diagnóstico de la pre-ez en ovinos y caprinos [en línea]. INTA EEA Bariloche. Argentina. www.biblioteca.org.ar/libros/210336.pdf
Boggio, J.C. 2005. Consejos y datos para la sincronización del estro en lanares con esponjas intravaginales [en línea]. Ergomix, Argentina. https://www.engormix.com/MA-ovinos/articulos/consejos-datos-sincronizacion-estro-t548/103-p0.html
Brand, T.S., Terblanche, S. & Jordaan, J.W. 2014. Conception rate and fecundity of Dohne Merino ewes in a continuous mating system. S. Afr. J. Anim. Sci 44(5): 64-69.
Catalano, R., González, C., Teruel, M., Cabodevila, J. & Callejas, S. 2005. Efecto del estado fisiológico y del porcentaje de raza Frisona sobre la respuesta reproductiva de ovejas en servicio de primavera. In. Vet. 7: 99-105.
Catalano, R., González, C., Teruel, M., Cabodevila, J. & Callejas, S. 2007. Efecto de diferentes dosis de gonadotrofina coriónica equina sobre la respuesta reproductiva de hembras ovinas con un tratamiento para inducción de celos [en línea]. In Vet. 9 (1): 11-17. http://www.scielo.org.ar/scielo.php?pid=S1668-34982007000100001&script=sci_arttext
Chacón, G., Abecia, J.A., Palacios, C. & Baselga, R. 2010. Determinación de progesterona mediante quimioluminiscencia en ganado ovino [en línea]. p 168-172 In XXXV Congreso de la SEOC. Set 22-25. Sociedad Espa-ola de Ovinotecnia y Caprinotecnia. Valladolid, Espa-a. http://www.google.co.cr/url?sa=t&source=web&cd=7&ved=0CEQQFjAG&url=http%3A%2F%2Fwww.seoc.eu%2Fdocs%2Fjornadas%2F35_jornadas_seoc.pdf&ei=ZUNmTsy
Clemente, O.N., Orihuela, A., Flores, F.I. & Aguirre, V. 2013. Citología y Análisis morfométrico de las células del epitelio vaginal durante el ciclo estral en ovejas de pelo (Ovis aries). Int. J. Morphol. 31 (3): 888-893. https://doi.org/10.4067/S0717-95022013000300017
Servicio Nacional de Salud Animal (SENASA). 2016. Dirección de Medicamentos Veterinarios: centro de consulta de MediVet [en línea]. Dirección de Medicamentos Veterinarios de SENASA. http://www.senasa.go.cr/medivet/WebForm2.aspx?id=ES-7-26-12-4601
Dogan, I. & Nur, Z. 2006. Different estrous induction methods during the non-breeding season in Kivircik ewes. Veterinární Medicina 51: 133-138.
Duggavathi, R. 2004. Dynamics and regulation of ovarian antral follicular waves in sheep. Ph.D. Thesis, University of Saskatchewan, Canada.
Espinoza R.R., Fraga, E.E. & Domínguez, M.M. 2003. Esponjas intravaginales manufacturadas con acetato de medroxiprogesterona para sincronizar el estro en ovinos de pelo [en línea]. p 50 In XXVII Congreso Nacional de Buiatría. Jun 10-14. Ammveb, México. http://ammveb.net/XXVII%20CNB/memorias/Reproduccion/Oral/htm/Trabajo_50_Esponjas_intravaginales_manufacturadas.htm
Fernández, A. 2007. Producción ovina quiere engordarse [en línea]. El Financiero. C.R. http://wvw.elfinancierocr.com/ef_archivo/2007/agosto/26/economia1202775.html
Fernández-Abella, D., Bonilla-Riera, C., Irabuena, O. & Sterla, S. 2003. Efecto del método de sincronización de celos y el manejo del semen conservado sobre la fecundidad ovina [en línea] Ovinos-Caprinos.com. Arg. http://www.ovinos-caprinos.com/FERTILIDAD/EFECTO%20DEL%20METODO%20DE%20SINCRONIZACION%20DE%20CELOS%20Y.pdf
Fukui, Y., Ishikawa, D., Ishida, N., Okada, M., Itagaki, R. & Ogiso, T. 1999. Comparison of fertility of estrous synchronized ewes with four different intravaginal devices during the breeding season. J. Reprod. Develop. 45: 337-343. https://doi.org/10.1262/jrd.45.337
Fukui, Y., Tetsuka, M., Akaikf, M., Machiyama, K. & Ono, H. 1987. Effects of types of vaginal sponge impregnated with progestogen on estrous induction and lambing rate in seasonally anestrous ewes. Anim. Reprod. Sci. 33: 181-187.
Gatti, M., Zunino, P. & Ungerfeld, R. 2011. Changes in the aerobic vaginal bacterial mucous load after treatment with intravaginal sponges in anoestrous ewes: Effect of medroxiprogesterone acetate and antibiotic treatment use. Reprod. Dom. Anim. 46: 205-208. https://doi.org/10.1111/j.1439-0531.2010.01626.x
Gibbons, A. & Cueto, M. 1995. Manual de inseminación artificial de la especie ovina [en línea]. INTA EEA Bariloche. Arg. http://inta.gob.ar/documentos/manual-de-inseminacion-artificial-en-la-especie-ovina/
Gibbons, A. & Cueto, M. 2004. Jornadas de inseminación artificial con semen fresco en ovinos [en línea]. INTA EEA Bariloche. Arg. http://www.inta.gov.ar/bariloche/info/documentos/animal/reproduc/ct-443.pdf
González, S. 2015. Determinación de la eficacia farmacológica y los efectos secundarios de una esponja intravaginal de fabricación casera elaborada con acetato de medroxiprogesterona para la sincronización del estro en ovinos de pelo. Tesis de Licenciatura en Medicina Veterinaria. Escuela de Medicina Veterinaria, Universidad Nacional, Heredia, Costa Rica.
Hernández-Olivos, J.L. 2010. Fertilidad en ovejas de pelo sincronizadas con acetato de medroxiprogesterona y gonadotropina coriónica equina. Tesis de Médico Veterinario Zootecnista. Universidad Veracruzana. Veracruz, Méx.
INEC. VI Censo Nacional Agropecuario. 2014. Censo Nacional Agropecuario 2014 [en línea]: Costa Rica total de fincas con ganado ovino por cantidad de animales y sexo según provincia. Instituto Nacional de Estadística y Censos, C.R http://inec.cr/agropecuario?title=&shs_term_node_tid_depth=All&field_periodo_tid=All&field_anio_documento_value%5Bvalue%5D%5Bdate%5D=
López-Crispín, J. 2010. Relación de hallazgos de citología vaginal exfoliativa y concentraciones séricas de progesterona dentro de un esquema de sincronización de estros en ovejas de pelo. Tesis de Médico Veterinario Zootecnista. Universidad Veracruzana. Veracruz, Méx.
Manes, J., Hozbor, F., Alberio, R. & Ungerfeld, R. 2014. Intravaginal placebo sponges affect negatively the conception rate in sheep. Small Rumin. Res. 120: 108-111. https://doi.org/10.1016/j.smallrumres.2014.05.006
Manes, J., Fiorentino, M.A., Kaiser, G., Hozbor, F., Alberio, R., Sanchez, E. & Paolicchi, F. 2010. Changes in the aerobical vaginal flora after treatment with different intravaginal devices in ewes. Small Rumin. Res. 94: 201-204. https://doi.org/10.1016/j.smallrumres.2010.07.021
Martemucci, G. & D’Alessandro, A.G. 2011. Synchronization of oestrus and ovulation by short time combined FGA, PGF2α, GnRH, eCG treatments for natural service of AI fixed-time. Anim. Reprod. Sci. 123: 32-39. https://doi.org/10.1016/j.anireprosci.2010.11.007
Martínez-Tinajero, J.J., Izaguirre-Flores, F., Sánchez-Orozco, L., García-Castillo, C.G., Martínez-Priego, G. & Torres-Hernández, G. 2007. Comportamiento reproductivo de ovejas barbados barriga negra sincronizadas con MPA y diferentes tiempos de aplicación de eCG durante la época de baja fertilidad. Rev. Cient. Fac. Cien. V XVII: 47-52.
Martínez- Tinajero, J.J., Sánchez, M.T., Torres-Hernández, G., Herrera-Haro, J.G., Bucio-Alanís, L., Rojo-Rubio, R. & Hernández-Martínez, J. 2008. Comportamiento reproductivo de ovejas F1 (Damara X Merino) sincronizadas con CIRD y dos tiempos de aplicación de GnRH. Universidad y Ciencia 24(3): 175-182.
Martins, G., Figueira, L., Penna, B., Brandᾶo, F., Varges, R., Vasconcelos, C. & Lilenbaum, W. 2009. Prevalence and antimicrobial susceptibility of vaginal bacteria from ewes treated witn progestin-impregnated intravaginal sponges. Small Rumin. Res. 81: 182-184. https://doi.org/10.1016/j.smallrumres.2008.12.003
Martins, L.T., dos Santos-Neto, P.C., Neto, S.G., Pereira-Rauber, L., Bertolini, M., Diniz-Viera, A. & Mezzalira, A. 2010. Microbiological and functional evaluation of an alternative device (OB®) for estrous synchronization in ewes. Cienc. Rural 40: 389-395. https://doi.org/10.1590/S0103-84782010000200021
McNeal, L. 1998. Ram breedeing soundness evaluation [en línea]. The Navajo Sheep Project. Utah. http://navajosheepproject.com/images/pdf/rams/bse.pdf
Menchaca, A., Pinzak, A. & Queirolo, D. 2005. Storage of ram semen at 5°C: effects of preservation period and timed artificial insemination on pregnancy rate in ewes. Anim. Reprod 3(2): 195-198.
Naim, P., Cueto, M. & Gibbons, A. 2009. Inseminación artificial a tiempo fijo con semen ovino refrigerado. Arch. Zootec. 58: 435-440. https://doi.org/10.4321/S0004-05922009000300012
Noakes, D.E., T.J. Parkinson & G.C.W. England (ed). 2001. Arthur’s Veterinary Reproduction and Obstetrics. 8th. ed. London, Saunders. 864 p.
Olivera-Muzante, J., Fierro, S., López, V. & Gil, J. 2011. Comparison of prostaglandin and progesterone-based protocols for time artificial insemination in sheep. Theriogenology 75: 1232-1238. https://doi.org/10.1016/j.theriogenology.2010.11.036
Penzzanite, L., Bridges, A., Neary, M. & Hutchens, T. [s.f.]. Breeding soundness examinations of rams and bucks [en línea]. Purdue University Cooperative Extension Service. United States. http://www.extension.purdue.edu/extmedia/AS/AS-599-W.pdf
Robinson, T.J., Quinlivan, T.D. & Baxter, C. 1968. The relationship between dose of progestagen and method of preparation of intravaginal sponges on their effectiveness for the control of ovulation in the ewe. J. Reprod. Fertil. 17: 471-483. https://doi.org/10.1530/jrf.0.0170471
Santos, I.W., Binsfeld, L.C., Weiss, R.R. & Kozicki, L.E. 2010. Fertility rates of ewes treated with medroxiprogesterone and injected with equine chorionic gonadotropin plus human chorionic gonadotropin in anoestrous season. Vet. Med. Int. 2010: 1-4. https://doi.org/10.4061/2010/978520
Scott, P.R. 2007. Sheep Medicine. Manson Publishing. London, UK. 336 p.
Silva, B.D.M., Sartori, R., Silva, T.A. Cardozo, D.M.M., Oliveira, M.A.L. & Neves, J.P. 2010. Estrus synchronization with prostaglandin F2α compared to progestogen treatment associated with equine chorionic gonadotropin (eCG) in Santa Ines breed ewes reared in Federal District, Brazil. Ci. Anim. Bras. 11(2): 417-424.
Simonetti, L., Blanco, M.R. & Gardón, J.C. 2000. Estrus synchronization in ewes treated with sponges impregnated with different doses of medroxiprogesterone acetate. Small Ruminant Res. 38: 243-247. https://doi.org/10.1016/S0921-4488(00)00160-7
Squires, E.J. 2003. Applied Animal Endocrinology. CABI Publishing. UK. https://doi.org/10.1079/9780851995946.0000
Thompson, J. & Meyer, H. 1994. Body condition scoring of sheep [en línea]. Oregon State University Extension Service. United States. http://ir.library.oregonstate.edu/xmlui/bitstream/handle/1957/14303/ec1433.pdf;jsessionid=88471CD05DC32B17C93CE09FD511076D?sequence=1
Ungerfeld, R., Suárez, G., Carbajal, B., Silva, L., Laca, M., Forsberg, M. & Rubianes, E. 2003. Medroxiprogesterone priming and response to the ram effect in Corriedale ewes during the nonbreeding season. Theriogenology 60: 35-45. https://doi.org/10.1016/S0093-691X(02)01302-X
Wulster-Radcliffe, M.C., Wang, S. & Lewis, G.S. 2004. Transcervical artificial insemination in sheeps: effects of a new transcervical artificial insemination instrument and traversing the cervix on pregnancy and lambing rates. Theriogenology. 62: 990-1002. https://doi.org/10.1016/j.theriogenology.2003.12.031
Zarwaki, M. 2001. Oestrous and twinning rate of Syrian Awassi ewes treated with progesterone and PMSG during the breeding season. New Zeal J. Agr. Res. 44: 159-163. https://doi.org/10.1080/00288233.2001.9513472
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