Molecular characterization of the Newcastle disease virus that caused an outbreak in backyard birds in Costa Rica in 2015
DOI:
https://doi.org/10.15359/rcv.41-1.1Keywords:
Newcastle disease, genotype V, backyard poultry, Costa RicaAbstract
Costa Rica gained its Newcastle Disease Virus NDV-free status with vaccination according to OIE proceedings in 1996, and its declaration as a country free of the velogenic, viscerotropic form of this disease (G/SPS/GEN/119) presented to the World Trade Organization (WTO) in 1999. On April 24th, 2015, SENASA (National Animal Health Service) attended a velogenic Newcastle disease outbreak that affected backyard chickens in a small town (Bellavista, Guanacaste) in the northern part of the country, near the Nicaraguan border. Sixty-five backyard birds died from a total of 84 exposed animals. Blood samples, cloacal swabs, tracheal swabs, cecal tonsils, lung and trachea tissues were collected for diagnosis at the National Veterinary Services Laboratory (LANASEVE). These samples were screened for Avian Influenza (AIV) and NDV. All samples were negative for Avian Influenza in ELISA test and RT-PCR. Serum samples were positive for NDV antibody by hemagglutination inhibition test, and tissue and swab samples were positive for NDV by conventional RT-qPCR targeting a 310 bp fragment of the virus fusion protein gene. The amino acid sequence of the protease cleavage site within the amplicon matched the sequence of a virulent strain (112RRQKRF117). The nucleotide sequence had a 98.7% identity and an e value of 4e-153 with a genotype V velogenic sequence from Belize (KF767467) and Honduras (JN872194) collected in 2008 and 2007, respectively, according to BLASTN. A total of 3604 backyard birds were euthanized in town and its surroundings (1 km), including 3495 chickens, 66 turkeys, 6 geese, and 37 ducks. The case was considered resolved, and OIE was notified in November 2015 following OIE guidelines. In April 2017, Costa Rica recovered its disease-free status through executive decree No. 40301-MAG.
References
Abd Elfatah, K. S., Elabasy, M. A., El-khyate, F., Elmahallawy, E. K., Mosad, S. M., El-Gohary, F. A., Abdo, W., Al-Brakati, A., Seadawy, M. G., Tahoon, A. E., & El-Gohary, A. E. (2021). Molecular Characterization of Velogenic Newcastle Disease Virus (Sub-Genotype VII.1.1) from Wild Birds, with Assessment of Its Pathogenicity in Susceptible Chickens. Animals, 11(2), 505. https://doi.org/10.3390/ani11020505
Agarwala, R., Barrett, T., Beck, J., Benson, D. A., Bollin, C., Bolton, E., Bourexis, D., Brister, J. R., Bryant, S. H., Canese, K., Cavanaugh, M., Charowhas, C., Clark, K., Dondoshansky, I., Feolo, M., Fitzpatrick, L., Funk, K., Geer, L. Y., Gorelenkov, V., … Zbicz, K. (2018). Database resources of the National Center for Biotechnology Information. Nucleic Acids Research, 46(D1), D8–D13. https://doi.org/10.1093/nar/gkx1095
Decreto 40301-MAG, (2017).
Baele, G., Lemey, P., & Vansteelandt, S. (2013). Make the most of your samples: Bayes factor estimators for high-dimensional models of sequence evolution. BMC Bioinformatics, 14(1), 85. https://doi.org/10.1186/1471-2105-14-85
Ballagi-Pordány, a, Wehmann, E., Herczeg, J., Belák, S., & Lomniczi, B. (1996). Identification and grouping of Newcastle disease virus strains by restriction site analysis of a region from the F gene. Archives of Virology, 141(February), 243–261. https://doi.org/10.1007/BF01718397
Bouckaert, R., Vaughan, T. G., Barido-Sottani, J., Duchêne, S., Fourment, M., Gavryushkina, A., Heled, J., Jones, G., Kühnert, D., De Maio, N., Matschiner, M., Mendes, F. K., Müller, N. F., Ogilvie, H. A., du Plessis, L., Popinga, A., Rambaut, A., Rasmussen, D., Siveroni, I., … Drummond, A. J. (2019). BEAST 2.5: An advanced software platform for Bayesian evolutionary analysis. PLOS Computational Biology, 15(4), e1006650. https://doi.org/10.1371/journal.pcbi.1006650
Cattoli, G., Susta, L., Terregino, C., & Brown, C. (2011). Newcastle disease a review of field recognition and current methods of laboratory detection. Journal of Veterinary Diagnostic Investigation, 23(4), 637–656. https://doi.org/10.1177/1040638711407887
Diel, D. G., da Silva, L. H. A., Liu, H., Wang, Z., Miller, P. J., & Afonso, C. L. (2012). Genetic diversity of avian paramyxovirus type 1: Proposal for a unified nomenclature and classification system of Newcastle disease virus genotypes. Infection, Genetics and Evolution, 12(8), 1770–1779. https://doi.org/10.1016/j.meegid.2012.07.012
Dimitrov, K. M., Abolnik, C., Afonso, C. L., Albina, E., Bahl, J., Berg, M., Briand, F. X., Brown, I. H., Choi, K. S., Chvala, I., Diel, D. G., Durr, P. A., Ferreira, H. L., Fusaro, A., Gil, P., Goujgoulova, G. V., Grund, C., Hicks, J. T., Joannis, T. M., … Wong, F. Y. K. (2019). Updated unified phylogenetic classification system and revised nomenclature for Newcastle disease virus. Infection, Genetics and Evolution, 74(June). https://doi.org/10.1016/j.meegid.2019.103917
Dimitrov, K. M., Ferreira, H. L., Pantin-Jackwood, M. J., Taylor, T. L., Goraichuk, I. V., Crossley, B. M., Killian, M. L., Bergeson, N. H., Torchetti, M. K., Afonso, C. L., & Suarez, D. L. (2019). Pathogenicity and transmission of virulent Newcastle disease virus from the 2018–2019 California outbreak and related viruses in young and adult chickens. Virology, 531(January), 203–218. https://doi.org/10.1016/j.virol.2019.03.010
Garcia, S. C., Navarro Lopez, R., Morales, R., Olvera, M. A., Marquez, M. A., Merino, R., Miller, P. J., & Afonsoa, C. L. (2013). Molecular epidemiology of newcastle disease in Mexico and the potential spillover of viruses from poultry into wild bird species. Applied and Environmental Microbiology, 79(16), 4985–4992. https://doi.org/10.1128/AEM.00993-13
Hall, T. A. (1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl. Acids. Symp., 41, 95–98. http://jwbrown.mbio.ncsu.edu/JWB/papers/1999Hall1.pdf
Hernandez-Divers, S. M., Villegas, P., Jimenez, C., Hernandez-Divers, S. J., Garcia, M., Riblet, S. M., Carroll, C. R., O’Connor, B. M., Webb, J. L., Yabsley, M. J., Williams, S. M., & Sanchez, S. (2008). Backyard chicken flocks pose a disease risk for neotropic birds in Costa Rica. Avian Diseases, 52(4), 558–566. https://doi.org/10.1637/8298-032808-Reg.1
León-Rodríguez, B., Vargas-Brenes, O., Guevara Soto, M., & Solano-Pereira, M. (2009). Análisis molecular de una cepa de virus de Newcastle de origen vacunal aislada a partir de un hisopado cloacal de aves sanas en Costa Rica - Molecular analysis of an isolated Newcastle disease virus strain obtained from cloacal swabs in healthy poultry fa. REDVET. Revista Electrónica de Veterinaria, 10(11), 1–19.
Merino, R., Villegas, H., Quintana, J. A., & Calderon, N. (2009). Characterization of Newcastle disease viruses isolated from chicken, gamefowl, pigeon and quail in Mexico. Veterinary Research Communications, 33(8), 1023–1030. https://doi.org/10.1007/s11259-009-9321-5
Miller, P. J., Decanini, E. L., & Afonso, C. L. (2010). Newcastle disease: Evolution of genotypes and the related diagnostic challenges. Infection, Genetics and Evolution, 10(1), 26–35. https://doi.org/10.1016/j.meegid.2009.09.012
Miller, P. J., Haddas, R., Simanov, L., Lublin, A., Rehmani, S. F., Wajid, A., Bibi, T., Khan, T. A., Yaqub, T., Setiyaningsih, S., & Afonso, C. L. (2015). Identification of new sub-genotypes of virulent Newcastle disease virus with potential panzootic features. Infection, Genetics and Evolution, 29(July), 216–229. https://doi.org/10.1016/j.meegid.2014.10.032
Miller, P. J., Kim, L. M., Ip, H. S., & Afonso, C. L. (2009). Evolutionary dynamics of Newcastle disease virus. Virology, 391(1), 64–72. https://doi.org/10.1016/j.virol.2009.05.033
Nguyen, L. T., Schmidt, H. A., Von Haeseler, A., & Minh, B. Q. (2015). IQ-TREE: A fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Molecular Biology and Evolution, 32(1), 268–274. https://doi.org/10.1093/molbev/msu300
Perozo, A. F., Merino, R., Afonso, C. L., Villegas, P., Calderon, N., Perozo, F., Merino, A. R., Afonso, B. C. L., Villegas, C. P., & Calderon, N. B. (2008). Biological and Phylogenetic Characterization of Virulent Newcastle Disease Virus Circulating in Mexico Biological and Phylogenetic Characterization of Virulent Newcastle Disease Virus Circulating in Mexico. Avian Diseases, 52(3), 472–479. https://doi.org/10.1637/1933-5334(2008)3
Perozo, F., Marcano, R., & Afonso, C. L. (2012). Biological and phylogenetic characterization of a genotype VII Newcastle disease virus from Venezuela: Efficacy of field vaccination. Journal of Clinical Microbiology, 50(4), 1204–1208. https://doi.org/10.1128/JCM.06506-11
Rambaut, A., Lam, T. T., Max Carvalho, L., & Pybus, O. G. (2016). Exploring the temporal structure of heterochronous sequences using TempEst (formerly Path-O-Gen). Virus Evolution, 2(1), vew007. https://doi.org/10.1093/ve/vew007
Spackman, E., Senne, D. A., Myers, T. J., Bulaga, L. L., Garber, L. P., Perdue, M. L., Lohman, K., Daum, L. T., & Suarez, D. L. (2002). Development of a real-time reverse transcriptase PCR assay for type A influenza virus and the avian H5 and H7 hemagglutinin subtypes. Journal of Clinical Microbiology, 40(9), 3256–3260. https://doi.org/10.1128/JCM.40.9.3256
Stäuber, N., Brechtbühl, K., Bruckner, L., & Hofmann, M. A. (1995). Detection of Newcastle disease virus in poultry vaccines using the polymerase chain reaction and direct sequencing of amplified cDNA. Vaccine, 13(4), 360–364. http://www.ncbi.nlm.nih.gov/pubmed/7793131
Suchard, M. A., Lemey, P., Baele, G., Ayres, D. L., Drummond, A. J., & Rambaut, A. (2018). Bayesian phylogenetic and phylodynamic data integration using BEAST 1.10. Virus Evolution, 4(1), 1–5. https://doi.org/10.1093/ve/vey016
Susta, L., Hamal, K. R., Miller, P. J., Cardenas-Garcia, S., Brown, C. C., Pedersen, J. C., Gongora, V., & Afonso, C. L. (2014). Separate evolution of virulent Newcastle disease viruses from Mexico and Central America. Journal of Clinical Microbiology, 52(5), 1382–1390. https://doi.org/10.1128/JCM.00066-14
Susta, L., Hamal, K. R., Miller, P. J., Cardenas-garcia, S., Brown, C. C., Pedersen, J. C., Gongora, V., & Afonso, L. (2014). Separate Evolution of Virulent Newcastle Disease Viruses from Mexico and Central America. J Clin Microbiol, 52(5), 1382–1390. https://doi.org/10.1128/JCM.00066-14
Veterinary Services, A. and P. H. I. (2019). Report on the Review of Costa Rica’s Animal Health Statuses (Issue August).
Zanetti, F., Berinstein, A., Pereda, A., Taboga, O., Carrillo, E., Zanetti, F., Berinstein, A. A., Pereda, A. B. A., Taboga, A. B. O., & Carrilloabc, E. (2005). Molecular Characterization and Phylogenetic Analysis of Newcastle Disease Virus Isolates from Healthy Wild Birds. Avian Diseases, 49(4), 546–550.
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