Effect of time on the stability of hematological variables in dog blood samples
Keywords:
whole blood, preservation, hemogram, time, caninesAbstract
The aim of this study was to assess the effect of the time of storage of dog blood samples. Hematological variables studied included: Hematocrit, hemoglobin, mean corpuscular hemoglobin concentration (MCHC), leukocyte and differential leukocyte counts. Samples were collected from 40 dogs of different breed, age, sex, and clinical condition treated at the Hospital for Small and Wild Species of the School of Veterinary Medicine (EMV) from Universidad Nacional in Costa Rica, during the period from August 2007 to May 2008. Blood samples were taken from the cephalic vein, using the vacuum system (Vacutainer ®) with disodium salt of ethylenediaminetetraacetic acid (EDTA) as an anticoagulant. Samples were analyzed in the Laboratory of Clinical Analysis at the EMV and were kept at a temperature of 39.2°F (4°C).Methodologies used included conventional manual methods. Each sample was analyzed four times; the first analysis was done during the first hour after sampling, and successively at 24, 48 and 72 hours. Statistical analysis (SAS ver.9.2) included the calculation of descriptive statistics
for each hematological variable, a comparison of means for each of the variables in the time, according to the data grouped in terciles in order to assess the possible effect of concentration and cellularity. Results showed minimal not significant differences over time; therefore, clinical or biological implications, and thus the possibility of establishing a wrong clinical diagnosis, is unlikely, given the stability of the hematological variables analyzed.
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