Tetraiodothyronine, triiodothyronine and cortisol serum levels in Costa Rican dogs using an immunoassay analyzer
Keywords:
canines, hormones, reference interval, AIA-360®Abstract
Hormonal reference intervals vary according the measurement method used and the geochemical environment where populations develop. Consequently, each lab must have its own reference intervals in order to interpret results properly. This paper was aimed at establishing a reference interval for total T4 (TT4), free T4 (FT4), total T3 (TT3) and cortisol in Costa Rican dogs, using the Tosoh Bioscience’s AIA-360® automatic analyzer. Blood serum from 122 randomly selected clinically healthy canines was used to determine reference intervals. Additionally, TT4, FT4 and TT3 were measured in 123 canines with suspected symptoms of hypothyroidism, and a low dose dexamethasone suppression test was performed in 12 canines with suspicious signs of hyperadrenocorticism. Samples were processed in the AIA-360® equipment after calibration curves were performed following manufacturer’s instructions. The reference interval was estimated based on the 3rd. and 97th. percentiles of healthy canines values: TT4 11.58 nmol/L – 50.19 nmol/L; FT4 6.18 pmol/L – 41.18 pmol/L; TT3 0.69 nmol/L – 1.64 nmol/L; cortisol 17.38 nmol/L – 174.64 nmol/L. FT4 concentration was statistically lower (p<0.05) in canines with suspected symptoms of hypothyroidism. Four of the canines with suspicious signs of hyperadrenocorticism did not suppress, while 7 suppressed at 4 hours after dexamethasone, but rebounded at 8 hours. Currently, a reference interval for TT4, FT4, TT3, and cortisol is available for Costa Rican canines using the AIA-360® analyzer.
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