In vitro determination of the effectiveness of entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae to control cattle tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae)
DOI:
https://doi.org/10.15359/rcv.35-1.3Keywords:
Rhipicephalus microplus, Metarhizium anisopliae, Beauveria bassiana, integrated tick control, entomopathogenic fungiAbstract
Using in stable artificial infestations of Bos taurus x Bos taurus calves to obtain sufficient engorged female ticks from Rhipicephalus microplus, in vitro assays were performed with strains of entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana to determine their pathogenicity and eventual use in control programs. Two strains of M. anisopliae and one of B. bassiana were cultured in the laboratory. Different conidial concentrations per milliliter were used for the assay. As indicators, the percentages of mortality and inhibition of oviposition were measured. BM-BL04 and BM-MSG06 strains were able to control the female adult population by over 96%. The combination of the BM-MSG06 and BM-BL04 strains decreased female oviposition by almost 100% in both cases, followed by the same strains, but applied individually. When performing the statistical analysis of the data (p <0.05), all strains obtained significant differences when compared to the control group. Regarding the hatching percentage analyzed in the additional assay, the BM-MSG06 strain successfully inhibited egg hatching of the engorged female, followed by the combination of these strains and then by the BM-BL04 strain.References
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